Objective: Explore contribution of ratio of xylem to phloem(RXP) to evaluate the quality of Radix Isatidis.
Methods: Antivirus activity and chemical compositions of xylem, phloem and Radix Isatidis of different RXP were determined by RBC agglutination test and unique chromatogram. Meanwhile, correlation between RXP and bioactivity,components was investigated.
Results: the activity of medical material of Radix Isatidis whose RXP was 1:2 or 1:1 is equal to that of phloem sample, while is stronger than that of cylem sample. There was a good consistency among the chemical figureprints of three samples (Radix Isatidis, xylem and phloem). When the RXP was 2:1, the medical material of Radix Isatidi and its xylem had the same activity. But the activity of phloem was not obvious. Their consistency of chemical fingerprint was bad, and the activity of Radix Isatidis which had RXP of 1:2 or 1:1 was better than that formed by xylem and phloem of 2:1. The Radix Isatidis of RXP of 1:2 or 1:1 had less similarity of chemical figureprint with that having RXP of 2:1.
Conclusion: The quality of Radix Isatidis made up by the various RXP had significant difference. Radix Isatidis whose RXP is less than 1:1 had good quality and better activity. As a characteristic parameter of biologic morpha, the RXP can be applied to identifying the quality of Radix Isatidis, and also provided a reference to evaluation of other medical material of roots.
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Virology
January 2025
College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, PR China. Electronic address:
Porcine reproductive and respiratory syndrome virus (PRRSV) is a viral infectious disease that can cause infection in pigs of different ages. The condition known as porcine reproductive and respiratory syndrome poses a serious risk to the world's pig business and results in significant financial losses. Fuzhengjiedu San (FZJDS) is a traditional Chinese medicine compound, the main components include:Radix Isatidis, Radix Astragali and Herba Epimedii.
View Article and Find Full Text PDFPoult Sci
November 2024
College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Road, Xiangfang District, Harbin 150030, PR China; Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, PR China. Electronic address:
Natural drugs possess exceptional pharmacological properties, yet their development is often hindered by a lack of clarity regarding the mechanisms of their pharmacological actions. Building on our previous research, we employed a co-infection model with Mycoplasma gallisepticum (MG) and Escherichia coli (E. coli) to investigate the pharmacological action of Radix Isatidis Mixtures (RIM).
View Article and Find Full Text PDFSci Rep
September 2024
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
Int J Biol Macromol
October 2024
College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China. Electronic address:
Interstitial nephritis is the primary cause of mortality in IBV-infected chickens. Our previous research has demonstrated that Radix Isatidis polysaccharide (RIP) could alleviate this form of interstitial nephritis. To explore the mechanism, SPF chickens and chicken embryonic kidney cells (CEKs) were pre-treated with RIP and subsequently infected with QX-genotype IBV strain.
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
July 2024
The pinoresinol-lariciresinol reductase (PLR), a crucial enzyme in the biosynthesis of lignans in plants, catalyzes a two-step reaction to produce lariciresinol and secoisolariciresinol. Lignans such as lariciresinol are the effective components of traditional Chinese medicine Radix Isatidis in exerting antiviral activity. In order to study the function of the key enzyme PLR in the biosynthesis of lariciresinol in , the original plant of Radix Isatidis, was cloned from , with a full length of 954 bp, encoding 317 amino acids.
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