AI Article Synopsis
- Protoplasts from two isolates each of Beauveria bassiana and Metarhizium anisopliae were isolated using enzymatic methods and fused with polyethylene glycol to create hybrid strains.
- Four successful fused protoplasts were regenerated on specific agar media, showing increased activity in proteinase and chitinase enzymes compared to the original isolates.
- The study utilized RAPD and RFLP techniques to analyze genomic DNA, highlighting the protoplast fusion method as an effective tool for identifying and studying the hybrids and their applications.
Article Abstract
Protoplasts were isolated from two isolates each of Beauveria bassiana and Metarhizium anisopliae using lysing enzymes. Intra- and intergeneric protoplast fusion has been carried out using 40% polyethylene glycol. The fused protoplasts of B. bassiana and M. anisopliae have been regenerated on Czapek-Dox agar media, and a total of four fusants were selected for further studies. An increase in proteinase and chitinase enzyme activity was recorded with all fusants as compared to the wild-type isolates. To understand the nature of recombination process, random amplification of polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) were carried out on genomic DNA of fused and wild-type isolates. The present study demonstrates the scope and significance of the protoplast fusion technique as a rapid consistent method for identification of B. bassiana and M. anisopliae fused and wild-type isolates based on the banding pattern of RAPD and RFLP that can be reliably used ahead for further applications on these species.
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| http://dx.doi.org/10.1007/s12223-011-0055-8 | DOI Listing |
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