Aim: To study the interaction between MAVS and p21 regulates MAVS-mediated pathway.
Methods: Firstly, we cloned full-length p21 gene by PCR. And then the interaction between MAVS and p21 was further demonstrated by co-immunoprecipitation. The regulation of MAVS-mediated pathway by p21 was analyzed by luciferase reporter assays.
Results: There are interaction between p21 and MAVS in 293T cells. p21 downregulates activity of MAVS.
Conclusion: It is shown that p21 inhibits MAVS-mediated activation of NF-κB and IFN-β by Luciferase reporter assays. Overall, these results laid the foundation for further investigating the function of p21 in MAVS-mediated pathway.
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