Detection of gene expression in mouse embryos and tissue sections.

Methods Mol Biol

Department of Genetics, Harvard Medical School, Boston, MA, USA.

Published: November 2011

Analysis of gene expression patterns is central to the study of embryonic development. This chapter details methods for detecting gene expression in whole mouse embryos and in tissue sections. The most commonly used methods available in mouse are described and include mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters.

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http://dx.doi.org/10.1007/978-1-61779-210-6_10DOI Listing

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