AI Article Synopsis
- This study measured the concentration of THCCOOH, a cannabis metabolite, in different hair types (pubic, axillary, beard, and head) from cannabis users.
- The research involved a complex process of hair sample preparation and analysis using gas chromatography tandem mass spectrometry (GC/MS/MS-NCI).
- Results showed that pubic hair had higher THCCOOH concentrations than head hair, but no significant difference in concentrations between head and body hair based on urine test results was found.
Article Abstract
In this study, the concentrations of 11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid (THCCOOH) in pubic, axillary and beard hair were measured and the correlation between the concentrations of THCCOOH in head and pubic hair from same cannabis users were evaluated. The papers on body hair analysis for THCCOOH were rarely found although police officers submit body hair as a complimentary specimen to forensic laboratories in case cannabis users had no hair. Head, pubic, axillary, and beard hair were collected. All hair samples were cut into 0.5mm segments and decontaminated with methanol, digested with 1 mL of 1M NaOH at 85 °C for 30 min and extracted in 2 mL of n-hexane:ethyl acetate (9:1) two times after adding 1 mL of 0.1N sodium acetate buffer (pH = 4.5) and 200 μL of acetic acid followed by derivatization with 50 μL of PFPA and 25 μL of PFPOH for 30 min at 70 °C. The extracts were analyzed using gas chromatography tandem mass spectrometry operating in negative chemical ionization mode (GC/MS/MS-NCI). We determined the concentrations of THCCOOH in both pubic and head hair. The concentrations of THCCOOH in pubic hair were higher than those in head hair. We also evaluated the concentrations of THCCOOH in body hair (pubic, axillary and beard hair) and head hair according to the positive/negative urine test results. There was no statistically significant difference in the concentrations of THCCOOH in head and body hair according to urine results.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.forsciint.2011.06.026 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Are Δ-Tetrahydrocannabinol and Its Major Metabolites Substrates or Inhibitors of Placental or Human Hepatic Drug Solute-Carrier Transporters?
Int J Mol Sci
November 2024
Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195, USA.
Article Synopsis
- Δ-Tetrahydrocannabinol (THC) is the main psychoactive ingredient in cannabis, and its usage among pregnant individuals is rising, leading to concerns about fetal exposure.
- Research indicates that fetal THC exposure is significantly lower than maternal levels due to a specific placental transporter that actively reduces THC transfer to the fetus, although the exact identity of this transporter remains unknown.
- In lab studies, it was discovered that THC and its metabolite THC-COOH acted as substrates for certain liver transporters but were not inhibitors, highlighting the complexity of THC metabolism and transport in the body.
A key factor in monitoring cannabis consumption trends through wastewater analysis: Partitioning of THCCOOH between the liquid and solid phase of influent wastewater.
Water Res
December 2024
Environmental and Public Health Analytical Chemistry, Research Institute for Pesticides and Water, University Jaume I, Castellón, Spain. Electronic address:
Current wastewater-based epidemiology (WBE) studies are predominantly focused on the analysis of urinary biomarkers present in the liquid phase of influent wastewater (IWW). This approach systematically underestimates less polar metabolites, such as cannabis biomarkers. These biomarkers can potentially sorb to and desorb from suspended particulate matter (SPM) present in IWW.
View Article and Find Full Text PDFInsight into the mechanism of adsorption and release of 11-nor-9-carboxy-Δ-tetrahydrocannabinol in sewage by modeling regional suspended particles for evaluation of the influence on monitoring of cannabis illicit abuse.
J Chromatogr A
September 2024
National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
The adsorption of 11-nor-9-carboxy-∆-tetrahydrocannabinol (THC-COOH) by the suspended particles in sewage makes it fail to accurately monitor cannabis abuse. In this work, the model sewage sample was prepared through equivalent mixing the sewage from 10 different sewage treatment plants in Guangdong province of China and used as a comprehensive representative for investigating the adsorption and release behavior of THC-COOH on the suspended particles under different temperature and pH. The solid-liquid distribution of THC-COOH in sewage depended strongly on the adsorption and release properties which were susceptible to the temperature and pH, specially adjusting pH to 11.
View Article and Find Full Text PDFAssessment of Δ9-THC and Δ9-THCCOOH bias, precision, and ionization suppression/enhancement between solid tissue homogenate and supernatant by LC-MS-MS.
J Anal Toxicol
October 2024
State of Maryland Office of the Chief Medical Examiner, 900 West Baltimore St., Baltimore, MD 21223, United States.
Liquid chromatography-triple quadrupole mass spectrometry (LC-MS-MS) assays are frequently utilized for screening and confirmatory purposes in the forensic toxicology laboratory. While these techniques are excellent for the targeted identification and quantitation of a wide variety of drug classes, validation and determining fit-for-purpose is a significant requirement for each method. In the USA, the American National Standards Institute and Academy Standards Board first edition of Standard 036 currently serves as a primary resource in forensic toxicology method validation and mandates that laboratories evaluate critical performance characteristics to help ensure the production of forensically defensible results.
View Article and Find Full Text PDFAcute Cannabis Administration Transiently Reduces Mitochondrial DNA in Young Adults: Findings from a Secondary Analysis of a Double-Blind, Placebo-Controlled, Randomized Clinical Trial.
Cannabis Cannabinoid Res
June 2024
Department of Pharmacology & Toxicology, Medical Sciences Building, University of Toronto, Toronto, Canada.
Cannabis is one of the world's most commonly used substances; however, many questions remain unanswered as to how cannabis impacts the body. Recently, there has been a resurgence of research into the effects of plant-derived cannabinoids on mitochondrial health. In particular, a number of studies implicate mitochondrial-Δ9-tetrahydrocannabinol (Δ9-THC) interactions with altered memory, metabolism, and catalepsy in mice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!