AI Article Synopsis
- The study examined how cell culture conditions, specifically the presence of serum and proteins, affect the characteristics and behavior of positively charged nanoparticles (NP(+)) in bronchial epithelial cells.
- The presence of serum and proteins reduced the surface charge of NP(+) but did not change their size, and this altered charge impacted how efficiently the nanoparticles were taken up by the cells.
- Despite showing some oxidative DNA damage at high doses, NP(+) did not demonstrate significant cytotoxicity or genotoxicity in standard assays, indicating that they may be reasonably safe for use in biological contexts.
Article Abstract
We used well-characterized and positively charged nanoparticles (NP(+)) to investigate the importance of cell culture conditions, specifically the presence of serum and proteins, on NP(+) physicochemical characteristics, and the consequences for their endocytosis and genotoxicity in bronchial epithelial cells (16HBE14o-). NP(+) surface charge was significantly reduced, proportionally to NP(+)/serum and NP(+)/BSA ratios, while NP(+) size was not modified. Microscopy studies showed high endocytosis of NP(+) in 16HBE14o-, and serum/proteins impaired this internalization in a dose-dependent manner. Toxicity studies showed no cytotoxicity, even for very high doses of NP(+). No genotoxicity was observed with classic comet assay while primary oxidative DNA damage was observed when using the lesion-specific repair enzyme, formamidopyrimidine DNA-glycosylase (FPG). The micronucleus test showed NP(+) genotoxicity only for very high doses that cannot be attained in vivo. The low toxicity of these NP(+) might be explained by their high exocytosis from 16HBE14o- cells. Our results confirm the importance of serum and proteins on nanoparticles endocytosis and genotoxicity.
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| http://dx.doi.org/10.1016/j.ijpharm.2011.07.014 | DOI Listing |
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