The equine herpesvirus 1 (EHV-1) negative regulatory IR2 protein (IR2P), an early 1,165-amino acid (aa) truncated form of the 1487-aa immediate-early protein (IEP), lacks the trans-activation domain essential for IEP activation functions but retains domains for binding DNA, TFIIB, and TBP and the nuclear localization signal. IR2P mutants of the N-terminal region which lack either DNA-binding activity or TFIIB-binding activity were unable to down-regulate EHV-1 promoters. In EHV-1-infected cells expressing full-length IR2P, transcription and protein expression of viral regulatory IE, early EICP0, IR4, and UL5, and late ETIF genes were dramatically inhibited. Viral DNA levels were reduced to 2.1% of control infected cells, but were vey weakly affected in cells that express the N-terminal 706 residues of IR2P. These results suggest that IR2P function requires the two N-terminal domains for binding DNA and TFIIB as well as the C-terminal residues 707 to 1116 containing the TBP-binding domain.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388945 | PMC |
| http://dx.doi.org/10.1016/j.virol.2011.06.023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prognosis of patients with Hodgkin lymphoma and indeterminate response to PD-1 inhibitor therapy: considerations for application of LYRIC criteria in real clinical practice.
Ann Hematol
December 2024
RM Gorbacheva Research Institute, Pavlov University, L'va Tolstogo Str. 6-8, 197022, St. Petersburg, Russia.
PD-1 inhibitors have shown unconventional response patterns in classic Hodgkin lymphoma (cHL). These include the phenomenon of pseudoprogression, highlighting the need for specialized response criteria such as the LyRIC, which stringened definitions for disease progression with introduction of indeterminate response category. Despite their potential utility, these provisional criteria are currently underutilized and require further refinement through clinical practice data collection.
View Article and Find Full Text PDFExploring the relationship between caffeine metabolism-related CYP1A2 rs762551 polymorphism and team sport athlete status and training adaptations.
Mol Biol Rep
July 2024
Sports Science Faculty, Lokman Hekim University, Ankara, 06510, Türkiye.
Background: This study aimed to achieve a dual objective: to compare the frequencies of CYP1A2 rs762551 genotypes between team sport athletes and a control group, and to determine the association between the rs762551 polymorphism and changes in physical performance after a six-week training program among elite basketball players.
Methods: The study encompassed an analysis of 504 individuals, comprising 320 athletes and 184 controls. For the Turkish cohort, DNA was isolated using the buccal swab method, and genotyping was conducted using the KASP technique.
Image restoration of degraded time-lapse microscopy data mediated by near-infrared imaging.
Nat Methods
February 2024
Morgridge Institute for Research, Madison, WI, USA.
Time-lapse fluorescence microscopy is key to unraveling biological development and function; however, living systems, by their nature, permit only limited interrogation and contain untapped information that can only be captured by more invasive methods. Deep-tissue live imaging presents a particular challenge owing to the spectral range of live-cell imaging probes/fluorescent proteins, which offer only modest optical penetration into scattering tissues. Herein, we employ convolutional neural networks to augment live-imaging data with deep-tissue images taken on fixed samples.
View Article and Find Full Text PDFUnveiling the regulatory mechanisms of salicylate degradation gene cluster in sp. strain X9.
Appl Environ Microbiol
October 2023
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Article Synopsis
- - This study builds on previous research showing that a specific gene cluster in *X9* strain helps degrade salicylate via a CoA-mediated pathway and introduces a new transcriptional regulator, CehR4, which works differently than other known regulators.
- - CehR4 forms an operon with the gentisyl-CoA thioesterase gene, while two other important genes for salicylate metabolism form another operon, and their overlapping promoters regulate their transcription.
- - The binding sites for CehR4 were identified, revealing how it controls the gene cluster involved in salicylate degradation, shedding light on its regulatory role and emphasizing the relevance of microbial degradation in reducing harmful aromatic compounds in
Variation within major internal repeats of KSHV in vivo.
Virus Evol
May 2023
Department of Microbiology, University of Washington, 960 Republican St, Seattle, WA 98109-4325, USA.
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma (KS), yet the viral genetic factors that lead to the development of KS in KSHV-infected individuals have not been fully elucidated. Nearly, all previous analyses of KSHV genomic evolution and diversity have excluded the three major internal repeat regions: the two origins of lytic replication, internal repeats 1 and 2 (IR1 and IR2), and the latency-associated nuclear antigen (LANA) repeat domain (LANAr). These regions encode protein domains that are essential to the KSHV infection cycle but have been rarely sequenced due to their extended repetitive nature and high guanine and cytosine (GC) content.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!