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Subcellular localization of transiently expressed fluorescent fusion proteins.
Methods Mol Biol
March 2014
Biomolecular Interaction Centre, School of Biological Sciences, The University of Canterbury, Christchurch, New Zealand.
The recent and massive expansion in plant genomics data has generated a large number of gene sequences for which two seemingly simple questions need to be answered: where do the proteins encoded by these genes localize in cells, and what do they do? One widespread approach to answering the localization question has been to use particle bombardment to transiently express unknown proteins tagged with green fluorescent protein (GFP) or its numerous derivatives. Confocal fluorescence microscopy is then used to monitor the localization of the fluorescent protein as it hitches a ride through the cell. The subcellular localization of the fusion protein, if not immediately apparent, can then be determined by comparison to localizations generated by fluorescent protein fusions to known signalling sequences and proteins, or by direct comparison with fluorescent dyes.
View Article and Find Full Text PDFPhylogeography of the ocellated skink Chalcides ocellatus (Squamata, Scincidae), with the use of mtDNA sequences: a hitch-hiker's guide to the Mediterranean.
Mol Phylogenet Evol
February 2010
Department of Biology, University of Crete, Vassilika Vouton, GR-71409 Irakleio, Crete, Greece.
We analyze geographic genetic variation in C. ocellatus to evaluate the influences of major climatic, paleogeographic and anthropogenic factors in its biogeographic history. Ninety four specimens from 61 populations were collected across all of its geographical range and analyzed based on partial mitochondrial sequences (cyt b, 12S, and ND1).
View Article and Find Full Text PDFEducating and empowering young girls in poor countries is part of the solution
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