Acorus calamus, an ethnomedicinally important plant, was investigated for its protecting activity against radiation induced DNA and membrane damage. The in vitro free radical scavenging activity of the extract (water:ethanol, 1:1) of A. calamus was studied by parameters viz DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, and superoxide radical scavenging activity. Membrane damage due to radiation exposure was measured as the peroxidation of lipids in terms of thiobarbituric acid reacting substance (TBARS). The in vitro DNA damage was monitored by assessing the radiation induced relaxation of supercoiled plasmid DNA (pBR322). Damage to cellular DNA induced by γ-radiation (6Gy) was monitored by alkaline single cell gel electrophoresis or comet assay in murine cells and human peripheral blood leukocytes. Enhancement of DNA repair mechanism was also monitored. The extract effectively scavenged free radicals in a concentration dependent manner. Presence of A. calamus extract during irradiation prevented peroxidation of membrane lipids in mouse liver homogenate. It helped to reduce the disappearance of the covalently closed circular (ccc) form of plasmid DNA following exposure to γ-radiation. Also the A. calamus extract effectively protected DNA from radiation induced strand breaks and enhanced the DNA repair process. Hence A. calamus extract can be used as a good source of natural radioprotecting agent.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.etap.2010.02.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anti-oxidants as Therapeutic Agents for Oxidative Stress Associated Pathologies: Future Challenges and Opportunities.
Free Radic Res
January 2025
Radiation Biology & Health Sciences Division, Bio-science Group, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India.
Free radicals have been implicated in the pathogenesis of cancer along with cardiovascular, neurodegenerative, pulmonary and inflammatory disorders. Further, the relationship between oxidative stress and disease is distinctively established. Clinical trials using anti-oxidants for the prevention of disease progression have indicated some beneficial effects.
View Article and Find Full Text PDFThe role of avocado plant and its derivatives in the management of diabetes mellitus: a natural approach to glycemic control.
J Diabetes Metab Disord
June 2025
Department of Physiology, Kampala International University, Western Campus, Ishaka, Uganda.
Purpose: Diabetes mellitus is a global health challenge that leads to severe complications, negatively impacting overall health, life expectancy, and quality of life. Herbal medicines, valued for their accessibility and therapeutic benefits with minimal side effects, have been promoted as potential treatments. Managing conditions like diabetes, characterized by free radical production and cytokine-driven inflammation, is vital due to the active components in plants that exert direct pharmacological effects.
View Article and Find Full Text PDFOptimization of Tratt pomace fermentation process and the effects of mono- and mixed culture fermentation on its chemical composition.
Front Nutr
December 2024
State Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China.
Background: Tratt pomace (RRTP) contains valuable components like polyphenols and polysaccharides, which have high utilization value. Fermentation is an effective technique for creating beneficial nutrients that can improve the taste, appearance, and nutritional benefits of foods. Nevertheless, there is a lack of research on the alterations in chemical composition of RRTP during fermentation.
View Article and Find Full Text PDFAntioxidant activity, phytochemical composition, and antitumor capacity of Amazonian fruits taperebá (Spondias mombin) and murici (Byrsonima crassifolia).
Arch Pharm (Weinheim)
January 2025
Program in Food and Nutrition, Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil.
The Amazon rainforest is renowned for its biodiversity and as a reservoir of edible and medicinal plants. The phytochemicals in murici and taperebá fruits serve as natural antioxidants, contributing to cultural preservation, ecosystem protection, and economic opportunities. However, limited scientific research on their composition and health benefits hinders their recognition as functional foods.
View Article and Find Full Text PDFTargeting mitochondrial function as a potential therapeutic approach for allergic asthma.
Inflamm Res
January 2025
Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, The First Dongguan Affiliated Hospital, College of Medical Technology, Guangdong Medical University, Dongguan, 523808, China.
Allergic asthma is a chronic complex airway disease characterized by airway hyperresponsiveness, eosinophilic inflammation, excessive mucus secretion, and airway remodeling, with increasing mortality and incidence globally. The pathogenesis of allergic asthma is influenced by various factors including genetics, environment, and immune responses, making it complex and diverse. Recent studies have found that various cellular functions of mitochondria such as calcium regulation, adenosine triphosphate production, changes in redox potential, and free radical scavenging, are involved in regulating the pathogenesis of asthma.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!