This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187145 | PMC |
| http://dx.doi.org/10.1128/AEM.05441-11 | DOI Listing |
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