Effects of vegetables-fruit extracts and indole-3-carbinol on stearic acid-modulated intercellular communication and cytochrome P450-IA activity.

Modulatory effects were investigated of extracts of a vegetables-fruit mixture and indole-3-carbinol (I3C) on stearic acid-modulated gap junctional intercellular communication (GJIC) and cytochrome P450-IA activity (EROD). In V79 cells, pure water and hexane extracts of a vegetables-fruit mixture and 25 μg/ml I3C significantly protected against decreased GJIC caused by 10 μM stearic acid. Furthermore, pure, 10× and 100× diluted vegetables-fruit extracts significantly maintained their capacity to induce EROD activity in Caco-2 cells, but only when these extracts were added to the cells in media already containing 500 μM stearic acid for 48 h. Stearic acid itself did not induce EROD activity. I3C (10, 25, and 50 μg/ml) clearly induced EROD activity in Caco-2 cells, irrespective of the order at which I3C and stearic acid were added to the cells. In conclusion, the present in vitro study showed that vegetables-fruit extracts and I3C modulate effects of stearic acid on intercellular communication and cytochrome P450-IA activity.