64 eight-week-old female rats were randomly divided into to 8 groups with four in medical treatment group and four in control group. The treatment groups were trained by progressive intensifying endurance swimming, and the medical-taken groups were fed with lycium for 5 weeks, and then the changes of the liver cellular free radical metabolism and ultrastructure were investigated. The effects of lycium and endurance training on liver cellular ultrastructure and free radical metabolism in rats were then explored. The results showed that exhaustive swimming time of rats could be extended by endurance training, and that exhaustive swimming time of rats could significantly be prolonged after taking lycium, and the ultrastructure of liver cell with taking lycium was more complete than that without taking lyceum. Lycium could cause significant increase of the activity of liver superoxide dismutase (SOD) and the ratio of SOD/MDA, and meanwhile cause decrease of the content of liver MDA. Conclusions can be drawn that lycium and endurance training can improve exercise capacity of rats, and lycium has certain protecting functions of the liver.
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Nat Commun
December 2024
Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY, USA.
Fibrolamellar Hepatocellular Carcinoma (FLC) is a rare liver cancer characterized by a fusion oncokinase of the genes DNAJB1 and PRKACA, the catalytic subunit of protein kinase A (PKA). A few FLC-like tumors have been reported showing other alterations involving PKA. To better understand FLC pathogenesis and the relationships among FLC, FLC-like, and other liver tumors, we performed a massive multi-omics analysis.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Vascular Surgery, Zhongshan Hospital, Fudan University, 200032, Shanghai, China.
Adverse aortic remodeling increases the risk of aorta-related adverse events (AAEs) after thoracic endovascular aortic repair (TEVAR) and affects the overall prognosis of aortic dissection (AD). It is imperative to delve into the exploration of prognostic indicators to streamline the identification of individuals at elevated risk for postoperative AAEs, and therapeutic targets to optimize the efficacy of TEVAR for patients with AD. Here, we perform proteomic and single-cell transcriptomic analyses of peripheral blood and aortic lesions, respectively, from patients with AD and healthy subjects.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Molecular and Medical Genetics, Oregon Health & Science University School of Medicine, Portland, OR, USA.
AAV vectors show promise for gene therapy; however, kidney gene transfer remains challenging. Here we conduct a barcode-seq-based comparison of 47 AAV capsids administered through different routes in mice, followed by individual validation. We find that local delivery of AAV-KP1, but not AAV9, via the renal vein or pelvis effectively transduces proximal tubules with minimal off-target liver transduction, while systemic AAV9, but not AAV-KP1, enhances proximal tubule and podocyte transduction in chronic kidney disease.
View Article and Find Full Text PDFAdv Sci (Weinh)
December 2024
Department of General Surgery, National-Local Joint Engineering Research Center of Biodiagnostic & Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China.
Liver metastasis is the main cause of cancer-related mortality. During the metastasis process, circulating carcinoma cells hardly pass through narrow capillaries, leading to nuclear deformation. However, the effects of nuclear deformation and its underlying mechanisms on metastasis need further study.
View Article and Find Full Text PDFOpen Vet J
November 2024
Directorate of Veterinary Medicine, Babylon, Iraq.
Background: Mycotoxins are considered one of the most important problems and threats that face poultry producers.
Aim: This study was conducted to investigate the pathological, hematological, and biochemical alterations in chickens fed on mycotoxins contamination ration.
Methods: 434 feed samples were collected from poultry farms operating in Babil Governorate/Iraq, where feed samples were collected over the course of 2023, and these samples were tested by direct competitive enzyme-linked immunosorbent assay to determine the level of mycotoxins.
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