Objective: To develop a standard enzyme-linked immunosorbent assay (ELISA) for the detection of bovine herpesvirus type 1 (BHV-1).
Methods: The assay was based on hyperimmune rabbit and guinea pig antisera raised against purified BHV-1. Polyethylene glycol precipitation and sucrose density gradient methods were adopted for viral concentration and purification. Antisera were raised using Freund's adjuvant followed by extraction of IgG of high purity.
Results: Optimum antisera dilutions as determined by titrations were chosen as 1:4 000, whereas the conjugate was used at 1:2 000 dilution. Using 95 clinical specimens, the ELISA test showed a sensitivity and specificity of 91.90 % and 93.10 %, respectively when compared to PCR. The cut-off value was fixed at 0.15 (A(490)) and a P/N ratio of >1.30 indicated a significant positive reaction.
Conclusions: The results have demonstrated that this ELISA could efficiently detect BHV-1 and can be used as an important diagnostic tool.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S1995-7645(11)60104-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
SMOC2 promotes microglia activity and neuroinflammation in Alzheimer's disease.
J Alzheimers Dis
January 2025
Department of Vascular Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Background: Alzheimer's disease (AD), the leading cause of dementia, is characterized by cognitive decline and the accumulation of amyloid-β (Aβ). It affects millions, with numbers expected to double by 2050. SMOC2, implicated in inflammation and fibrosis, may play a role in AD pathogenesis, particularly in microglial cell function, offering a potential therapeutic target.
View Article and Find Full Text PDFHypertensive disorders of pregnancy affected thyroid hormone synthesis via endoplasmic reticulum stress in preterm infant rats.
Heliyon
January 2025
Department of Neonatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Background: Maternal hypertensive disorders of pregnancy (HDP) was associated with increased risk of congenital hypothyroidism in preterm infants, but its underlying mechanisms remain unclear.
Objective: To investigate the possible mechanisms by which intrauterine exposure to HDP affects thyroid hormone synthesis in preterm infant rats.
Methods: preterm infant rats were obtained by Caesarean section delivery from the L-NAME group and Control groups which was induced by L-NAME and saline, respectively.
Navigating herpes simplex encephalitis: Family medicine perspectives from Jharkhand, India.
J Family Med Prim Care
December 2024
Department of Microbiology, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India.
Background: Herpes simplex encephalitis (HSE), caused by herpes simplex virus (HSV) is the most common cause of sporadic encephalitis that often presents as an emergency case of acute or sub-acute nature associated with poor prognosis. Early suspicion and prompt diagnostic testing with adequate antiviral therapy can only reduce morbidity and mortality associated with the disease. This study aims to evaluate the role of serological and molecular diagnosis of encephalitis caused by HSV 1 and 2 for timely detection of the disease.
View Article and Find Full Text PDFUp-regulation of miR-490-3p improves learning/memory disability of sevoflurane exposure by relieving neuroinflammation.
Toxicol Res (Camb)
January 2025
Department of Anaesthesia and Surgery, Shengli Oilfield Central Hospital, Dongying 257034, China.
Our study focused on the potential mechanism of microRNA-490-3p (miR-490-3p) on learning/memory disability of rats resulting from sevoflurane (Sev). The rat model of cognitive dysfunction was established by infection with miR-490-3p mimic and Sev-exposure. Morris water maze and open field test assay were used for the assessment of cognitive deficits.
View Article and Find Full Text PDFComparison of GastroPanel and GENEDIA in Diagnosing Infection and Gastric Lesions.
J Cancer Prev
December 2024
Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
Serological tests for needs local validation as the diagnostic accuracy may vary depending on the prevalence of . . This study examined the diagnostic performance of two ELISA, GastroPanel (GastroPanel ELISA; Biohit Oyj) and GENEDIA (GENEDIA .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!