AI Article Synopsis
- A new cell assay system allows for testing Bacillus thuringiensis delta-endotoxins at high pH levels without harming target cells.
- The assay is efficient, requiring just 1 microliter of sample per dose, and provides quantitative, rapid, and sensitive results.
- It has a threshold toxicity dose of 24 pg protein for B. thuringiensis subsp. kurstaki HD-73 and is effective for detecting inhibitory antibodies and beta-exotoxin.
Article Abstract
A cell assay system was developed that allows Bacillus thuringiensis delta-endotoxins activated at high pH (10.5) to be tested in vitro without causing alkaline injury to target cells. The assay is carried out on a lawn of gel-suspended cells, requires only 1 microliter of sample per dose, and is quantitative, rapid, and sensitive. The threshold dose for toxicity of B. thuringiensis subsp. kurstaki HD-73 with IPRI-CF-1 cells was 24 pg protein. The assay is also very useful for identifying antibodies which inhibit toxicity and for detecting beta-exotoxin.
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| http://dx.doi.org/10.1016/0022-2011(90)90106-g | DOI Listing |
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