Herpesvirus type 1 could be propagated most efficiently in cultured fetal neurons, to a lesser extent in NG108-15 neurohybridoma cells, and with the lowest titer in glial cells. Herpesvirus type 2 could not be cultured in neurohybridoma cells, and in fetal neurons a titer 100-fold lower than for herpesvirus type 1 was obtained. Cells infected with herpesvirus type 1 were used in an infectivity assay for acyclovir dose-response studies. The ED50 values were 7.4 nmol/l for fetal neurons, 180 for neurohybridoma cells, and 275 nmol/l for glial cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000150163 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Calcium and cAMP signaling induced by gamma-hydroxybutyrate receptor(s) stimulation in NCB-20 neurons.
Neuroscience
April 2010
Equipe Stéroïdes, Neuromodulateurs et Neuropathologies, Unité de Physiopathologie et Médecine Translationnelle, EA-4438, Faculté de médecine, Université de Strasbourg, Strasbourg, France.
The NCB-20 neurohybridoma cells differentiated with dibutyryl-cyclic-AMP represent an interesting model to study several components of the gamma-hydroxybutyrate (GHB) system in brain. In particular, an active Na(+)-dependent uptake and a depolarization-evoked release of GHB is expressed by these cells, together with high affinity specific binding sites for this substance. However, only little is known about cellular mechanisms following GHB receptor(s) stimulation in these neurons.
View Article and Find Full Text PDFA disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells.
FEBS Lett
September 1999
Institute of Legal Medicine, University of Hamburg, Germany.
Conjugates between anti-tetanus F(ab')2 fragments and the (37-72) fragment of the HIV Tat protein were taken up by chromaffin cells, NG108-15 neurohybridoma cells and Rev-2-T-6 lymphoma cells. The uptake could not be inhibited by competition with (37-72)Tat, but was reduced in the presence of metabolic inhibitors or at low temperature. The disulfide as well as the thioether conjugate were translocated to the cytoplasmic space, but only the disulfide conjugate moderately restored the stimulated transmitter release inhibited by tetanus toxin.
View Article and Find Full Text PDFNeurochemical and electrophysiological evidence for the existence of a functional gamma-hydroxybutyrate system in NCB-20 neurons.
Neuroscience
October 1998
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, UPR 416 CNRS, Centre de Neurochimie, Strasbourg, France.
Clonal neurohybridoma NCB-20 cells express a valproate-insensitive succinic semialdehyde reductase activity that transforms succinic semialdehyde into gamma-hydroxybutyrate. This activity (1.14+/-0.
View Article and Find Full Text PDFAcidification of the cytosol inhibits the uptake of tetanus toxin in NG108-15 and NBr-10A neurohybridoma cells.
Naunyn Schmiedebergs Arch Pharmacol
May 1996
Department of Toxicology, Medical School of Hannover, Germany.
The influence of cytosol acidification on the uptake of two-chain tetanus toxin (TeTX)1 by neurohybridoma cells NG 108-15 and NBr-10A was investigated with two established techniques, the NH4Cl pulse method and the pH-clamp method. With the former, the extracellular pH is maintained at its physiological value, but is set to different values with the latter. Acidification of the cytoplasm with an NH4Cl pulse retarded the uptake of TeTX by both NG 108-15 and NBr-10A cells.
View Article and Find Full Text PDFEffect of acyclovir on herpes simplex virus infected neuronal, glial, and neurohybridoma cells in vitro.
Intervirology
February 1991
Deutsche Wellcome GmbH, Burgwedel, FRG.
Herpesvirus type 1 could be propagated most efficiently in cultured fetal neurons, to a lesser extent in NG108-15 neurohybridoma cells, and with the lowest titer in glial cells. Herpesvirus type 2 could not be cultured in neurohybridoma cells, and in fetal neurons a titer 100-fold lower than for herpesvirus type 1 was obtained. Cells infected with herpesvirus type 1 were used in an infectivity assay for acyclovir dose-response studies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!