Chinese hamster ovary (CHO) cells are the primary platform for commercial expression of recombinant therapeutic proteins. Obtaining maximum production from the expression platform requires optimal cell culture medium (and associated nutrient feeds). We have used metabolite profiling to define the balance of intracellular and extracellular metabolites during the production process of a CHO cell line expressing a recombinant IgG4 antibody. Using this metabolite profiling approach, it was possible to identify nutrient limitations, which acted as bottlenecks for antibody production, and subsequently develop a simple feeding regime to relieve these metabolic bottlenecks. This metabolite profiling-based strategy was used to design a targeted, low cost nutrient feed that increased cell biomass by 35% and doubled the antibody titer. This approach, with the potential for utilization in non-specialized laboratories, can be applied universally to the optimization of production of commercially important biopharmaceuticals.

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.23269DOI Listing

Publication Analysis

Top Keywords

metabolite profiling
12
cho cells
8
antibody production
8
production
5
metabolite
4
recombinant
4
profiling recombinant
4
recombinant cho
4
cells designing
4
designing tailored
4

Similar Publications

Identification of novel serum lipid metabolism potential markers and metabolic pathways for oral cancer: a population-based study.

BMC Cancer

January 2025

Department of Epidemiology and Health Statistics, Fujian Provincial Key Laboratory of Environment Factors and Cancer, School of Public Health, Fujian Medical University, 1 Xueyuan Road, Fujian, 350108, China.

Objective: This study aims to identify potential lipid biomarkers and metabolic pathways associated with oral cancer (OC). Then to establish and evaluate disease classification models capable of distinguishing OC patients from healthy controls.

Methods: A total of 41 OC patients and 41 controls were recruited from a hospital in Southeast China to examine the serum lipidomics by Ultra-high Performance Liquid Chromatography Q Exactive Mass Spectrometry (UHPLC-QE-MS).

View Article and Find Full Text PDF

Profiling of secondary metabolites within Fragaria sp. (strawberry), Rubus sp. (raspberries and blackberries), and Ribes sp.

View Article and Find Full Text PDF

Plastic materials are ubiquitous, leading to constant human exposure to plastic additives such as plasticizers. There is growing evidence that plasticizers may contribute to obesity due to their disruptive effects on metabolism. Alternatives like diisononylcyclohexane-1,2-dicarboxylate (DINCH) are replacing traditional phthalates such as di-(2-ethylhexyl) phthalate (DEHP), which are now banned due to their proven harmful health effects.

View Article and Find Full Text PDF

Background: Lung cancer is the deadliest disease globally, with more than 120,000 diagnosed cases and more than 75,000 deaths annually in Japan. Several treatment options for advanced lung cancer are available, and the discovery of biomarkers will be useful for personalized medicine. Using metabolome analysis, we aimed to identify biomarkers for diagnosis and treatment response by examining the changes in metabolites associated with lung cancer progression.

View Article and Find Full Text PDF

Wild tomato species exhibit natural insect resistance, yet the specific secondary metabolites and underlying mechanisms governing the resistance remain unclear. Moreover, defense expression dynamically adapts to insect herbivory, causing significant metabolic changes and species-specific secondary metabolite accumulation. The present study aims to identify the resistance-related metabolites in wild tomato accessions that influence the defense mechanism against whitefly (Bemisia tabaci Asia II 7) and leafminer (Phthorimaea absoluta).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!