Oxidative DNA damage induced by a Copper(II)-1,10-phenanthroline-L-serine complex in the presence of rutin.

The capacity of the ternary complex copper(II)-1,10-phenanthroline-L-serine ([Cu-Phen-Ser]) to induce double-strand scission of DNA was explored by agarose-gel electrophoresis. It was found that the complex exhibited remarkable activity to damage DNA in the presence of rutin. Analysis of the UV and fluorescence spectra clearly demonstrated that the complex was bound to DNA by intercalation. Further, the occurrence of 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, after the treatment of DNA by the complex in presence of rutin was evidenced by an electrochemical method. Finally, the mechanism of oxidative damage to double-stranded DNA by the [Cu-Phen-Ser] complex in the presence of rutin was discussed.