We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/nmeth.1652 | DOI Listing |
Publication Analysis
Top Keywords
light-sheet microscopy
16
live imaging
8
two-photon scanned
8
scanned light-sheet
8
microscopy
5
deep fast
4
fast live
4
imaging two-photon
4
light-sheet
4
microscopy implemented
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!