Objective: To develop a HPLC method for the determination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.
Method: The separation was performed at 30 degrees C on an Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-water(45:55). The detection wavelength was 250 nm.
Results: The standard curve for evodiamine is linear in the range of 0.0510-0.560 microg, the average recovery was 97.5% (RSD 1.0%). The standard curve for rutacarpine is linear in the range of 0.0508-0.559 microg, the average recovery was 98.7% (RSD 1.5%).
Conclusion: The method is specific, accurate and stable. It can be used for detemination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Simultaneous determination of six alkaloids and one monoterpene in rat plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetic study after oral administration of a Chinese medicine Wuji Pill.
J Chromatogr B Analyt Technol Biomed Life Sci
May 2012
Department of Chemistry, School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
A simple and sensitive method for simultaneous determination of seven active constituents, jatrorrhizine, berberine, coptisine, palmatine, evodiamine, rutacarpine and paeoniflorin, from a Chinese medicine Wuji Pill in rat plasma was developed based on a liquid chromatography and tandem mass spectrometry method. The separation of these seven compounds was carried out on a Shiseido CAPCELL PAK C(18) column using a mobile phase consisting of acetonitrile (containing 0.1% formic acid and water (containing 0.
View Article and Find Full Text PDF[Determination of evodiamine and rutacarpine in zhuyu hewei zhitong capsules by HPLC].
Zhongguo Zhong Yao Za Zhi
April 2011
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Objective: To develop a HPLC method for the determination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.
Method: The separation was performed at 30 degrees C on an Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-water(45:55).
[Determination of evodiamine and rutaecarpine of compound Wuzhuyu cataplasm in plasma by SPE-HPLC: application to its pharmacokinetics].
Zhongguo Zhong Yao Za Zhi
November 2009
Institute of Chinese Meteria Maddica, China Academy of Chinese Medical Sciense, Beijing 100700, China.
Objective: To establish a SPE-HPLC method for the determination and pharmacokinetic study of evodiamine and rutacarpine in rat plasma.
Method: A Kromasil C18 column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-water-tetrahydrofuran-acetic acid (51:48:1:0.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!