Objective: To evaluate the performance of locked nucleic acid (LNA) probe Real-time polymerase chain reaction (PCR) in the detection of Aspergillus fumigatus (A. fumigatus).
Methods: All clinically cultured isolates of Aspergillus at our hospital were identified by morphology and DNA sequencing assay. The experimental group consists of A. fumigatus (n = 48) while the control group was made up of A. flavus (n = 55), A. versicolor (n = 16), A. nidulans (n = 10), A. sydowii (n = 5) and A. parasiticus (n = 1). The clinical samples consisted of A. fumigatus sinusitis tissue (n = 20) and bronchoalveolar lavage fluid (n = 1). DNA was extracted from all samples. A. fumigatus β-tubulin gene was targeted with LNA probe Real-time PCR assay. LNA probe Real-time PCR was evaluated with regards to specificity, efficiency, linear dynamic range in PCR amplification and limits of detection.
Results: All clinical samples were positively amplified. The specificity was 100% and the PCR efficiency 98.2%. Linear dynamic range was at least six orders of magnitude and the limit of detection 2.5 pg.
Conclusion: LNA probe Real-time PCR is a promisingly accurate assay of rapidly detecting A. fumigatus practically and cost-efficiently.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
How Much Data Are Enough? Toward Statistically Robust Adhesion Experiments by Atomic Force Microscopy.
Langmuir
January 2025
Department of Physical Chemistry II, University of Bayreuth, Universitaetsstrasse 30, Bayreuth 95447, Germany.
Direct force measurements by atomic force microscopy (AFM) have become an indispensable analytical tool in the last decades. Force measurements have been widely used for adhesion measurements, often in combination with the colloidal probe technique. For the latter technique, a colloidal particle is attached to the end of an AFM cantilever, proving great flexibility in terms of colloid/surface interaction to be studied.
View Article and Find Full Text PDFA miniaturized colorimetric-photoacoustic dual-mode device for point-of-care testing of ascorbic acid and ascorbate oxidase.
Anal Chim Acta
March 2025
The Radiology Department of Shanxi Provincial People's Hospital Affiliated to Shanxi Medical University, Taiyuan, 030001, China. Electronic address:
Background: Real-time and rapid detection of ingredients in food has important significance for food safety. However, traditional detection methods not only require bulky and costly instruments but also are often based on single-mode analysis, limiting their accuracy and applications in point-of-care testing. Herein, an integrated and miniaturized dual-mode device based on colorimetric and photoacoustic (PA) principles is developed, using Au@Ag nanoparticles (Au@AgNPs) as signal probe and ascorbic acid (AA) and ascorbate oxidase (AAO) as analytes.
View Article and Find Full Text PDFDetection of biogenic amines using a ylidenemalononitrile enamine-based fluorescence probe: Applications in food quality control.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046 China. Electronic address:
Biogenic amines (BAs) are a class of nitrogen-containing natural organic compounds. Elevated levels of BAs are a reliable indicator of food spoilage and pose a significant risk to human health. Thus, the development of real-time sensors for BAs monitoring is crucial.
View Article and Find Full Text PDFPutrescine is a kind of physical diamine that is closely related to food deterioration and food quality safety. This study employs a novel fiber optic biosensor based on S-tapered and waist extension techniques, as well as localized surface plasmon resonance (LSPR), to detect putrescine accurately. The gold nanoparticles (AuNPs) are fixed on the fiber to excite LSPR.
View Article and Find Full Text PDFInterleukin-17A and Interleukin-17F Gene Polymorphisms in Egyptian Patients with Chronic Hepatitis C and Hepatocellular Carcinoma.
Asian Pac J Cancer Prev
January 2025
Department of Biochemistry, Biotechnology Research Institute, High Throughput Molecular and Genetic laboratory, Center for Excellences for Advanced Sciences, National Research Centre, Dokki, Giza, Egypt.
Objective: Interleukin IL-17A and IL-17F are critical cytokines involved in inflammatory processes. Genetic variations in IL-17A and IL-17F might be linked to chronic hepatitis C (CHC) and an increased risk of hepatocellular carcinoma (HCC), a cancer associated with long-term inflammation. This study aims to examine the relationship between specific polymorphisms in IL-17A (rs2275913) and IL-17F (rs763780) and their association with HCV-related HCC in an Egyptian population.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!