This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.
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http://dx.doi.org/10.1007/978-1-61779-207-6_26 | DOI Listing |
Food Chem
January 2025
State Key Laboratory for Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China. Electronic address:
Heavy metals and mycotoxins are important contaminants in food pollution. Sensitive, reliable, and rapid detection of heavy metals and mycotoxins is crucial for human health. In this work, imidazole-functionalized aggregation-induced emission (AIE) molecule tetra-(4-pyridylphenyl) ethylene (TPPE) was used as a precise and specific probe for Ag detection, with a limit of detection (LOD) of 0.
View Article and Find Full Text PDFChembiochem
January 2025
University of Teramo: Universita degli Studi di Teramo, Veterinary Medicine, Piano d'Accio snc, 64100, Teramo, ITALY.
In this study, we employed a novel fluorescent probe, RO7304924-which selectively targets cannabinoid 2 receptor (CB2R)-to assess the lateral mobility of CB2R within the plasma membrane of Chinese hamster ovary cells stably expressing a functional, untagged receptor variant. Utilizing confocal fluorescence recovery after photobleaching (FRAP), we quantified the diffusion coefficient and mobile fraction of CB2R, thereby demonstrating the efficacy of RO7304924 as an innovative tool for elucidating the dynamics of this major endocannabinoid-binding G protein-coupled receptor. Our present findings highlight the potential of combining advanced ligand-based fluorescent probes with FRAP for future investigations into the biochemical details of CB2R mobility in living cells, and its impact on receptor-dependent cellular processes.
View Article and Find Full Text PDFMikrochim Acta
January 2025
Key Laboratory of Eco-Functional Polymer Materials of the Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, Gansu, China.
A pentafluoropropionic acid-functionalized fluorescent metal-organic framework material (UiO-66-NH-PFPA) is prepared by a simple post-synthetic modification (PSM) strategy for the sensitive and selective detection of dichloran (DCN). The results of fluorescence experiments demonstrate that the sensitivity of UiO-66-NH-PFPA (limit of detection, LOD = 0.478 μM) to DCN is nearly 10.
View Article and Find Full Text PDFAnal Methods
January 2025
Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, 695581, India.
The neuronal tau peptide serves as a key biomarker for neurodegenerative diseases, specifically, Alzheimer's disease, a condition that currently has no cure or definitive diagnosis. The methodology to noninvasively detect tau levels from body fluids remains a major hurdle for a rapid and simple diagnostic approach. Thus, developing new detection methods for sensing tau protein levels is crucial.
View Article and Find Full Text PDFFood Chem
January 2025
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address:
The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared.
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