Fluorescence recovery after photobleaching.

Methods Mol Biol

Faculty of Life Sciences, The University of Manchester, Manchester, UK.

Published: November 2011

This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.

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http://dx.doi.org/10.1007/978-1-61779-207-6_26DOI Listing

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