The sewage-associated real-time quantitative PCR (qPCR) assays BacHum and HF183 SYBR were compared for specificity against local fecal sources. Both assays were equally sensitive to sewage, but BacHum showed substantially more false-positive results for cat, dog, gull, and raccoon feces.
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http://dx.doi.org/10.1128/AEM.00239-11 | DOI Listing |
PLoS Negl Trop Dis
January 2025
ISGlobal, Barcelona, Spain.
Background: The Lihir Islands of Papua New Guinea, located in an area with high burden of malaria and hosting a large mining operation, offer a unique opportunity to study transmission. There, we investigated human and vector factors influencing malaria transmission.
Methods: In 2019, a cross-sectional study was conducted on 2,914 individuals assessing malaria prevalence through rapid diagnostic tests (RDT), microscopy, and quantitative PCR (qPCR).
PLoS One
January 2025
Department of Clinical Support Services, Division of Laboratory and Pathology Medicine, Uganda Cancer Institute, Kampala, Uganda.
The detection of Estrogen Receptor (ER), Progesterone Receptor (PR), and Human epidermal growth factor receptor 2 (HER-2) is important for the stratification of breast cancer and the selection of therapeutic modalities. This study aimed to determine the quantitative expression of ER, PR and HER-2 using Immunohistochemistry and their correlation with quantitative baseline Ct values measured using Quantitative Polymerase Chain Reaction (PCR). This study also assessed the use of fresh breast tissue biopsies preserved in RNAlater solution in the quantitative detection of these receptors using PCR technique.
View Article and Find Full Text PDFMol Biotechnol
January 2025
Hangzhou Vocational and Technical College, Hangzhou, 310018, China.
Azaleas (Rhododendron simsii) are popular ornamental woody plants known for their bright colors; however, very limited studies have been reported on the process of flower petal pigmentation. In this study, we found significant differences in the anthocyanin contents of petals from different colored azaleas, and the results of quantitative real-time PCR indicated that the R2R3 MYB genes, RsMYB12, RsMYB90, and RsMYB123, showed significant expression changes during the petal coloration in azalea petals; therefore, we hypothesized that RsMYB12, RsMYB90, and RsMYB123 might involve in the coloring process of azalea petals by regulating anthocyanin synthesis. This work provides insights into the underlying mechanisms of petal pigmentation in R.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Alzheimer's Disease Cooperative Study (ADCS), University of California, San Diego, La Jolla, CA, USA.
Background: Evaluating sex differences in modifiable risk factors for Alzheimer's disease could provide valuable information regarding the mechanisms by which these factors confer risk. Physical activity is a risk factor that has been shown to positively impact both telomere length, a marker of cellular age, and cognition. The goal of this study was to evaluate whether telomere length mediates the association between physical activity and cognition differently by sex.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Department of Neurology, Mayo Clinic, Rochester, MN, USA.
Background: Despite recent FDA approvement of disease-modifying treatments that reduce Aβ, the identification of novel therapeutic strategies that could delay the Alzheimer's disease (AD) development are needed. We identified and developed novel small molecule compounds that mildly inhibit mitochondrial complex I (MCI). Chronic treatment with a tool compound CP2 in 4 mouse models of familial AD was efficacious protecting against synaptic dysfunction and memory impairment, improving brain energetics and cognitive performance, reducing levels of human pTau and Ab.
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