AI Article Synopsis
- α-Dystroglycan (α-DG) is important for cell stability, and its N-terminal domain (α-DG-N) is secreted by cultured cells into their surrounding environment.
- A monoclonal antibody was created to study α-DG-N, which revealed significant amounts of this protein in human cerebrospinal fluid (CSF).
- The presence of α-DG-N in CSF, characterized as a sialylated glycoprotein with specific glycan types, suggests it may play unknown roles in the nervous system.
Article Abstract
α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and investigated the secretion of α-DG-N in human cerebrospinal fluid (CSF). Interestingly, we found that a considerable amount of α-DG-N was present in CSF. α-DG-N in CSF was a sialylated glycoprotein with both N- and O-linked glycan. These observations suggest that secreted α-DG-N may be transported via CSF and have yet unidentified effects on the nervous system.
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| http://dx.doi.org/10.1016/j.bbrc.2011.06.150 | DOI Listing |
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