We determined the prevalence and mean intensity of Trichinella sp. infection in coyotes from six counties in Oklahoma and one in northern Texas. Tongues from 77 coyotes were examined using histology and artificial tissue digestion. Histological examination showed a prevalence of 3.9% (3 of 77) whereas the prevalence was 6.5% (5 of 77) based on artificial digestion of 5.0 g of muscle from coyote tongues. One sample was positive for Trichinella sp. on histology but negative by artificial digestion. Combining data from both diagnostic techniques showed that six of 77 (7.8%) coyotes were infected with Trichinella spp. The mean intensity of Trichinella sp. larvae ranged from 0.2 to 66.2 with an average of 16.0 larvae per gram (LPG) of tongue. Genotyping results demonstrated that the coyotes were infected with Trichinella murrelli. This is the first report of T. murrelli infection in coyotes in Oklahoma. T. murrelli had previously been isolated from coyotes in Texas.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.vetpar.2011.06.001 | DOI Listing |
Vet Parasitol Reg Stud Reports
January 2025
Minnesota Department of Natural Resources, Wildlife Health Program, 5463 West Broadway, Forest Lake, MN 55025, USA.
During a survey for other parasitic infections, tongue and limb muscles of wild bobcats (Lynx rufus) were examined microscopically from a sample of 190 Minnesota bobcats. One batch (n = 50) was studied in 2015, yielding no cases of Trichinella when examined only with hematoxylin and eosin (HE) staining of <25 mg of tissue. In a 2024 cohort (n = 140), this method was paired with a compression of ∼5 g of tissue.
View Article and Find Full Text PDFJ Parasitol
December 2024
Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania 16802.
During a survey for Sarcocystis infections in Pennsylvania in wild canids, muscles from the tongue and limb were examined microscopically for sarcocysts. Between 9 February 2024 and 11 February 2024, muscle samples were collected from 76 coyotes, 46 gray foxes, and 21 red foxes from Pennsylvania hunter harvested animals. Around 5 g of muscle was examined microscopically by compression between a glass slide and coverslip.
View Article and Find Full Text PDFJ Parasitol
September 2024
United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350.
Trichinella murrelli Pozio and La Rosa, 2000, is the primary sylvatic trichinellid encountered in temperate North America. During a survey for Sarcocystis in wild canids, a single worm matching the morphology of encapsulated Trichinella was observed in a muscle tissue squash from a gray fox male originating from Pennsylvania. The worm was photographed and then separated from the host tissue by artificial digestion, and genomic DNA was extracted from the worm.
View Article and Find Full Text PDFVet Parasitol Reg Stud Reports
September 2024
United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705-2350, USA.
Trichinella infections have been eliminated from pork where pigs are raised in biosecure facilities, but wildlife infections persist. Trichinella murrelli is the primary zoonotic species in wild carnivores in the United States, having been identified in several species of omnivores and carnivores. Here, we document its occurrence in seven of 21 (33.
View Article and Find Full Text PDFParasit Vectors
December 2023
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Background: Amplicon-based next-generation sequencing (NGS) has rapidly gained popularity as a powerful method for delineating taxa in complex communities, including helminths. Here, we applied this approach to identify species and genotypes of zoonotic nematodes of the Trichinella genus. A known limitation of the current multiplex PCR (mPCR) assay recommended by the International Commission on Trichinellosis is that it does not differentiate Trichinella nativa from T.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!