Background: In barley, starch occurs in the form of granules with bimodal size distribution. Enzymatic hydrolysis of the starch granule is one of the most important reactions occurring during malting and mashing. Previous studies revealed the discrepancies in the assumption that barley varieties with better malting qualities should have a higher A/B (large/small starch granules) ratio. This led us to focus our attention on detailed analysis of two barley varieties, Jersey and Tolar, both with high malting quality but significantly differing in A/B (1.28 and 0.66, respectively), were chosen for more detailed analysis in the actual work. In this study, the capacity of gravitational field flow fractionation (GFFF) to monitor amylolysis of the starch granules was investigated.
Results: Isolated starch granules from these two barley cultivars were treated with amylases. The changes in starch granule size and bimodal distribution were studied by GFFF. Simultaneously, free sugars released during enzymatic digestion were observed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The changes in the fractogram and in the mass spectra reflect a correlation with the progress of enzymatic hydrolysis.
Conclusion: The results show the interest in utilization of GFFF as a simple and cheap method for monitoring changes in the distribution of the starch granule size during amylolysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jsfa.4518 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural, physicochemical and in vitro digestibility of white kidney bean protein-corn starch complexes under various heat treatments.
Food Res Int
January 2025
College of Food Science and Engineering, Changchun University, Changchun, Jilin 130022, China. Electronic address:
The presence of exogenous protein can delay starch digestion. However, systematic studies on the effects of protein on starch digestion under various heat treatments still need to be completed. In this study, the effects of exogenous protein and heat treatments on corn starch digestibility were investigated.
View Article and Find Full Text PDFInsights into the regulation mechanisms of dual hydrothermal treatment on the structure and digestive characteristics of A- and B-type wheat starch granules.
Food Res Int
January 2025
State Key Laboratory of Crop Stress Adaptation and Improvement, College of Agriculture, Henan University, Kaifeng 475004, China; Food Laboratory of Zhongyuan, Luohe 462300, China. Electronic address:
Hydrothermal treatment is a physical modification technology to alter starch structures for the production of resistant starch (RS). However, the underlying regulation mechanism of the multiscale structure and digestive properties of starch by dual hydrothermal synergistic treatment remains unclear. To solve this problem, A- and B-type wheat starch granules (AWS and BWS) were separated and subjected to toughening and heat-moisture synergistic treatment (THT) with various moisture content (10 %, 15 %, 20 %, 25 %).
View Article and Find Full Text PDFNatural starches suitable for 3D printing: Rhizome and seed starch from Millettia speciosa champ, a non-conventional source.
Carbohydr Polym
March 2025
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China. Electronic address:
The demand for exploring and investigating novel starches for various applications has been high, yet starches abundant in Millettia speciosa Champ (M. speciose) plants have barely been studied. This study aims to investigate the multiscale structure and physicochemical properties, especially good hot-extrusion 3D printability of M.
View Article and Find Full Text PDFButyrylated modification of corn starch alleviates autism-like behaviors by modulating 5-hydroxytryptamine metabolism and gut-brain neural activity.
Carbohydr Polym
March 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China; School of Food Science and Technology, Jiangnan University, Wuxi, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China; (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, China. Electronic address:
This study was conducted to elucidate the effects of different degrees of substitution (DS) on the properties of propionylated and butyrylated starches and to investigate their efficacy and mechanisms in ameliorating autism-like phenotypes. Fourier transform infrared spectra of propionylated and butyrylated starches revealed the presence of the CO absorption peak at 1730 cm. Additionally, as the DS increased, the surface of the starch granules became rougher, and the crystallinity decreased.
View Article and Find Full Text PDFCharacterization of a novel type 4 resistant starch from tapioca and its obesity-preventive effects through gut microbiota modulation in high-fat diet-treated mice.
Int J Biol Macromol
January 2025
Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Road, Taichung 40227, Taiwan. Electronic address:
The rising pandemic of obesity has received significant attention. Yet, more safe and effective targeted strategies must be used to mitigate its impact on individual health and the global disease burden. While the health benefits of resistant starch (RS) are well-documented, the role of RT-90 (a phosphate-modified tapioca RS containing 90.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!