One emphasis of the Gibbs Conference on Biothermodynamics is the value of thermodynamic measurements for understanding behaviors of biological systems. In this study, the correlation between thermodynamic measurements of in vitro DNA binding affinity with in vivo transcription repression was investigated for two transcription repressors. In the first system, which comprised an engineered LacI/GalR homolog, mutational changes altered the equilibrium constant for binding DNA. Changes correlated with altered repression, but estimates of in vivo repressor concentration suggest a ≥25-fold discrepancy with in vitro conditions. In the second system, changes in ligand binding to BirA altered dimerization and subsequent DNA occupancy. Again, these changes correlate with altered in vivo repression, but comparison with in vitro measurements reveals a ~10-fold discrepancy. Further analysis of each system suggests that the observed discrepancies between in vitro and in vivo results reflect the contributions of additional equilibria to the transcription repression process.
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http://dx.doi.org/10.1016/j.bpc.2011.06.005 | DOI Listing |
Sci Rep
January 2025
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
Plastic pollution and global warming are widespread issues that lead to several impacts on aquatic organisms. Despite harmful studies on both subjects, there are few studies on how temperature increases plastics' adverse effects on aquatic animals, mainly freshwater species. So, this study aims to clarify the potential impact of temperature increases on the toxicological properties of polyvinyl chloride nano-plastics (PVC-NPs) in Nile tilapia (Oreochromis niloticus) by measuring biochemical and oxidative biomarkers.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Univ Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes), UMR 6226, F-35000 Rennes, France. Electronic address:
The lack of understanding of polyplexes stability and their dissociation mechanisms, allowing the release of DNA, is currently a major limitation in non-viral gene delivery. One proposed mechanism for DNA-based polyplexes dissociation is based on the electrostatic interactions between polycations and biological polyanions, such as glycosaminoglycans (GAGs). This work aimed at investigating whether GAGs such as heparin, chondroitin sulphate and hyaluronic acid promote the dissociation of PEI/DNA polyplexes.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2025
A green facile method was developed to synthesize the carbon quantum dots from barberry, a native plant, as a new carbon source. The synthesis strategy is a simple one-step hydrothermal process without requiring hazardous chemical reagents. The spherical structure of b-CDs with an average particle size of 3.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Institute for Sustainability, Energy and Environment, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
Critical source areas (CSAs) can act as a source of phosphorus (P) in surface waters by releasing soil P to porewater during frequent rainfall events. The extent of P release under short-term, frequent submergence has not been systematically studied in CSAs in New Zealand. A study was conducted to explore the potential of three contrasting dairy and sheep/beef farm soils (Recent, Pallic and Allophanic soils) to release P to porewater and pondwater under short-term and frequent submergence.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2025
Key Laboratory of High-temperature and High-pressure Study of the Earth's Interior, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081 Guizhou, China. Electronic address:
High-pressure and high-temperature Raman spectra of natural pyromorphite, vanadinite and mimetite were measured up to 11 GPa and 973 K, respectively. No phase transition was observed within the temperature and pressure ranges in this study. Raman modes for pyromorphite, vanadinite and mimetite vary with temperature or pressure linearly.
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