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Development of a novel and affordable point-of-care kit for rapid detection of urea and glucose adulteration in cow milk.
Anal Methods
January 2025
Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science - Pilani, Hyderabad Campus, Hyderabad, Telangana 500078, India.
The increasing global population has raised the demand for cow milk, leading to its adulteration with harmful substances, including urea and glucose, that cause damage to humans when consumed regularly. Hence, this study started with predicting urea and glucose toxicity using ProTox-III software, wherein the results revealed that urea belongs to class IV with an LD value of 6350 mg kg and glucose belongs to class VI with an LD value of 23 000 mg kg. Then, a qualitative colorimetric kit and Fourier-transform infrared (FTIR) spectroscopy were used for the preliminary detection of urea and glucose in cow milk.
View Article and Find Full Text PDFEffectiveness of salt priming and plant growth-promoting bacteria in mitigating salt-induced photosynthetic damage in melon.
Photosynth Res
January 2025
Horticulture Department of Agriculture Faculty, Selcuk University, Konya, Turkey.
Seed priming and plant growth-promoting bacteria (PGPB) may alleviate salt stress effects. We exposed a salt-sensitive variety of melon to salinity following seed priming with NaCl and inoculation with Bacillus. Given the sensitivity of photosystem II (PSII) to salt stress, we utilized dark- and light-adapted chlorophyll fluorescence alongside analysis of leaf stomatal conductance of water vapour (G).
View Article and Find Full Text PDFTransformation-Guided Genome Mining Provides Access to Brominated Lanthipeptides.
Org Lett
January 2025
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
Natural product biosynthesis is nature's tinkering ground for developing new enzymes that can achieve chemical transformations that are outside the purview of traditional chemical catalysis. Herein we describe a genome mining approach that leads to the discovery of a halogenase that regioselectively brominates a tryptophan side chain indole for a macrocyclic peptide substrate, enabling downstream chemical arylation by Suzuki-Miyaura coupling. The halogenase was found to prefer a macrocyclic peptide substrate over a linear peptide.
View Article and Find Full Text PDFPseudopterosin Biosynthesis: Unravelling a Decades Old Problem in Animal Specialized Metabolism.
J Am Chem Soc
January 2025
Department of Medicinal Chemistry, University of Utah, 30 South 2000 East, Salt Lake City, Utah 84112, United States.
Soft corals are prolific producers of terpenoids, such as pseudopterosins. The exact biosynthetic pathway of these anti-inflammatory diterpene glycosides has eluded the scientific community for decades. Using a forward genetic approach, we have identified, cloned, and expressed the key genes involved in pseudopterosin biosynthesis.
View Article and Find Full Text PDFDihydropyrimidine dehydrogenase polymorphisms in patients with gastrointestinal malignancies and their impact on fluoropyrimidine tolerability: Experience from a single Italian institution.
World J Gastrointest Oncol
January 2025
Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples 80131, Campania, Italy.
Background: Fluoropyrimidines are metabolized in the liver by the enzyme dihydropyrimidine dehydrogenase (DPD), encoded by the gene. About 7% of the European population is a carrier of gene polymorphisms associated with reduced DPD enzyme activity.
Aim: To assess the prevalence of polymorphisms and their impact on fluoropyrimidine tolerability in Italian patients with gastrointestinal malignancies.
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