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Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA). | LitMetric

AI Article Synopsis

Article Abstract

The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263338PMC
http://dx.doi.org/10.1111/j.1574-6941.2011.01166.xDOI Listing

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