The transposable element Tdd-3 from D. discoideum has been described originally in 1984 (Poole and Firtel, 1984). Additional copies of this element were discovered in the course of a recent study on tRNA gene organization in D. discoideum. Five out of 24 independently isolated tRNA genes proved to be associated with Tdd-3 elements. The surprising observation that all the elements always occurred within the 3'-flanking sequences of the Dictyostelium tRNA genes suggested the possibility of a general position specific integration of Tdd-3 elements upon transposition. Therefore we isolated additional Tdd-3 elements from various genomic D. discoideum libraries in order to test this hypothesis. Several new Tdd-3 elements were found associated with various tRNA genes. Additionally we identified Tdd-3 elements organized in tandem array or in association with RED (Repetitive Element of Dictyostelium), another repetitive element recently identified by our laboratory. In all cases a B-box equivalent of the eukaryotic gene-internal RNA polymerase III promoter was identified upstream of all Tdd-3 elements.
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Non-LTR retrotransposons with unique integration preferences downstream of Dictyostelium discoideum tRNA genes.
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Institut für Molekulare Biotechnologie, Jena, Germany.
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Mol Gen Genet
April 1998
Institut für Pharmazeutische Biologie, Universität Frankfurt/M. (Biozentrum), Germany.
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December 1991
Institut für Biochemie, Medizinische Fakultät Universität Erlangen-Nürnberg, F.R.G.
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Institut für Biochemie, Medizinische Fakultät, Universität Erlangen-Nürnberg, FRG.
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Department of Biology, Utah State University, Logan 84322-5500.
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