The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.
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http://dx.doi.org/10.1128/AEM.00359-11 | DOI Listing |
Curr Med Mycol
May 2024
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background And Purpose: Onychomycosis is a common fungal infection that affects the nails, caused by various fungal agents. Moreover, yeast onychomycosis has increased in recent years. Yeast isolates might not be identified at the species level by conventional methods, whereas molecular methods can identify yeast isolates more accurately.
View Article and Find Full Text PDFCurr Med Mycol
May 2024
Department of Microbiology, Sri Ramachandra Medical College & Research Institute, SRIHER, Porur, Chennai 600116, India.
Background And Purpose: is the third most commonly isolated species from candidemia patients admitted to Indian intensive care units. Outbreak of infection and emergence of fluconazole resistance associated with this particular species has been increasingly documented since 2018. Worldwide data has documented that Y132F substitution in the gene is the predominant fluconazole resistance mechanism among .
View Article and Find Full Text PDFInt J Ophthalmol
December 2024
Molecular Biology and Genetics Department, Liaquat University of Medical and Health Sciences, Jamshoro 76090, Pakistan.
Aim: To find out the association of secreted protein acidic and rich in cysteine (SPARC)-related modular calcium binding 2 () gene variants rs2255680 and rs13208776 with genotypic and phenotypic characteristics in both familial and non-familial primary open angle glaucoma (POAG) patients.
Methods: A total of 212 POAG patients, comprising 124 familial and 88 non-familial, were enrolled. For genotyping the variant rs2255680, amplification refractory mutation system (ARMS)-polymerase chain reaction (PCR) method and PCR-restriction fragment length polymorphism (PCR-RFLP) were utilized for analyzing rs13208776 variant.
Biol Methods Protoc
October 2024
One Health Research Group, Facultad de Ciencias de la Salud, Universidad de Las Américas, Quito, 170124, Ecuador.
We present a rapid high-throughput DNA extraction method for use with EDTA-anticoagulated blood using silicon dioxide (SiO) powder in a guanidine-HCl solution, hereinafter referred to as "Glassmilk." The method utilizes a 96-well deep-well plate, enabling DNA extraction from 96 samples in under 3 h. The method integrates cell lysis, washing, elution, and DNA storage within the same well, eliminating the need for DNA transfer.
View Article and Find Full Text PDFZhonghua Yi Xue Yi Chuan Xue Za Zhi
December 2024
Department of Neonatology, Shangqiu First People's Hospital, Shangqiu, Henan 476000, China.
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