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Multitude of morphological dynamics of giant multilamellar vesicles in regulated nonequilibrium environments. | LitMetric

Multitude of morphological dynamics of giant multilamellar vesicles in regulated nonequilibrium environments.

Langmuir

Department of Basic Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 Japan.

Published: August 2011

AI Article Synopsis

  • * A new perfusion device for light microscopy allows researchers to flexibly control environmental conditions and observe individual phospholipid GVs over long periods.
  • * This device was applied to study the dynamics of giant multilamellar vesicles (GMVs), revealing various solubilization pathways influenced by surfactant concentration and GMV composition, which could help in understanding cellular morphology.

Article Abstract

Lipid giant vesicles (GVs) exhibit biologically relevant morphological dynamics such as growth and division under certain conditions without any sophisticated molecular machineries employed by the current organisms. Nonequilibrium conditions are essential for the emergence of dynamic behaviors, which are normally generated by the addition of stimulating materials or by the change of some physical conditions. Therefore, an experimental method that allows flexible control of external conditions is desirable. Here we report a new and simple perfusion device for light microscopy observation that simultaneously realizes such control and tracking of individual phospholipid GVs for the long-term. We apply this device to the study of the morphological dynamics of POPC-based giant multilamellar vesicles (GMVs) under a monotonic and gradual increase of surfactant concentration; thereby we reveal the existence of multiple pathways in the slow solubilization processes, whose frequencies depend on the compositions of GMVs. This perfusion device would offer an unprecedented control of external conditions in the studies of GVs and might help us characterize the physicochemical origins of rich morphological dynamics of living cells.

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Source
http://dx.doi.org/10.1021/la2018456DOI Listing

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