Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489282 | PMC |
| http://dx.doi.org/10.1126/science.1207773 | DOI Listing |
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