Many nanoscale systems are known to emit light intermittently under continuous illumination. In the fluorescence of single semiconductor nanoparticles, the distributions of bright and dark periods ('on' and 'off' times) follow Lévy statistics. Although fluorescence from single-quantum dots and from macroscopic quantum dot ensembles has been studied, there has been little study of fluorescence from small ensembles. Here we show that blinking nanorods (NRs) interact with each other in a cluster, and the interactions affect the blinking statistics. The on-times in the fluorescence of a NR cluster increase dramatically; in a cluster with N NRs, the maximum on-time increases by a factor of N or more compared with the combined signal from N well-separated NRs. Our study emphasizes the use of statistical properties in identifying the collective dynamics. The scaling of this interaction-induced increase of on-times with number of NRs reveals a novel collective effect at the nanoscale.
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http://dx.doi.org/10.1038/ncomms1357 | DOI Listing |
Alzheimers Dement
December 2024
University of Virginia, Charlottesville, VA, USA.
Background: Tau phosphorylated at threonine-217 (tau), which can be measured in plasma and CSF using antibodies, is one of the most promising early biomarkers for Alzheimer's disease (AD). Little was known, however, about the cellular and subcellular distributions of tau, how those levels change during disease progression, the factor(s) that provoke tau accumulation, or functional consequences of tau phosphorylation at T217. This study addressed all of those issues.
View Article and Find Full Text PDFSoft Matter
December 2024
William A. Brookshire Department of Chemical and Biomolecular Engineering, University of Houston, 4226 Martin Luther King Boulevard, Houston, Texas, 77204-4004, USA.
We investigate the dynamics of tracer nanoparticles in bulk supercooled nanoparticle matrices using confocal microscopy. We mix fluorescent (tracer) and undyed (matrix) charged-stabilized polystyrene nanoparticles with tracer-to-matrix particle size ratios = 0.34, 0.
View Article and Find Full Text PDFBiochem Biophys Rep
September 2024
Institute of Information and Communication Technologies at the Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Block 2, Sofia, 1113, Bulgaria.
Although antimicrobial peptides are considered one of the most promising alternatives to conventional antibiotics given the alarming increase in bacterial multidrug resistance, many aspects of their mechanism of action remain unclear, in particular the emergence and role of collective phenomena such as the spontaneous formation of nano-sized unstructured objects (clusters) and their effects on the biodynamics. We study this process using two novel peptides from the mucus of the garden snail as an example to reveal its dynamics and bioactivity implications through coordinated in silico and in vitro techniques - molecular dynamics simulations, UV-Vis and fluorescence spectroscopy, and antibacterial activity tests against two representative bacterial strains - one gram-negative ( 3458) and one gram-positive (). The results obtained confirm the impact of the aggregation processes of the peptides on their biological activity and provide insight into possible synergies in their action.
View Article and Find Full Text PDFEJNMMI Res
November 2024
Department of Radiology and Radiological Science, The Russell H. Morgan, Johns Hopkins School of Medicine, Baltimore, MD, USA.
bioRxiv
November 2024
Preventive and Restorative Dent. Sci., San Francisco, CA.
In this study, we performed synchrotron-based micro-X-ray fluorescence (μ-XRF) imaging of elements Zn and S, and X-ray absorption near edge spectroscopy (XANES) coupled with μ-XRF for identification of Zn and S species in the condylar zones of a rat temporomandibular joint (TMJ). Histologic localization of Zn and hypoxia-inducible factor-1α (HIF-1α) were mapped using an optical microscope. These data were visually correlated with μ-XRF and XANES data to provide insights into plausible biological S-species in Z-enriched condylar zones of a rat TMJ.
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