AI Article Synopsis
- Centrosomes are crucial for cell division and cilium formation, consisting of centrioles and a protein network known as pericentriolar material (PCM).
- The protein Sas-4 acts as a scaffold for cytoplasmic complexes (S-CAP) that include CNN, Asl, and D-PLP, which are vital for PCM assembly and stability of nascent procentrioles.
- Mutations in Sas-4 can lead to centrosomes with reduced PCM, and the study demonstrates that Sas-4 is essential for the initial assembly of PCM prior to its attachment to the centrosome.
Article Abstract
Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole. When Sas-4 is absent, nascent procentrioles are unstable and lack PCM, and functional centrosomes are not generated. When Sas-4 is mutated, so that it cannot form S-CAP complexes, centrosomes are present but with dramatically reduced levels of PCM. Finally, purified S-CAP complexes or recombinant Sas-4 can bind centrosomes stripped of PCM, whereas recombinant CNN or Asl cannot. In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677532 | PMC |
| http://dx.doi.org/10.1038/ncomms1367 | DOI Listing |
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