We generated single-frequency pulses at kilowatt peak power from an all-fiber Tm-doped master oscillator power amplifier system, which is the first report of this kind (to the best of our knowledge) of a laser in the 2 μm region. Compared with the laser linewidth of seed pulses, spectral broadening by a factor of 3 was observed with the amplified pulses. This was attributed to self-phase modulation in passive pigtail fibers of the components (isolator and wavelength division multiplexing) that were placed after the fiber amplifier. The short pulse width (~7 ns) of the kilowatt-level pulses prevents an onset of stimulated Brillouin scattering in the long fiber. When launching the pulses into several-meter single-mode fiber, significant nonlinear spectral broadening occurs due to modulation instability in the fiber. This reaction is beneficial for generation of a mid- and long-wavelength IR supercontinuum in nonlinear IR fibers.
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http://dx.doi.org/10.1364/OL.36.002293 | DOI Listing |
Acta Biomater
January 2025
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address:
Photothermal therapy (PTT) is a promising treatment strategy for malignant tumors. Photothermal agents which can achieve efficient photothermal conversion in the NIR-II region plays crucial roles in this remedy. Here, we report one type of thermo-responsive gold nanorod vesicles USGRV-17-AAG for combined NIR-II photothermal therapy and chemotherapy of solid tumors.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
July 2023
Maastricht MultiModal Molecular Imaging (M4i) Institute, Division of Imaging Mass Spectrometry (IMS), Maastricht University, 6229 ER Maastricht, The Netherlands.
We discuss the design, development, and evaluation of an Orbitrap/time-of-flight (TOF) mass spectrometry (MS)-based instrument with integrated UV photodissociation (UVPD) and time/mass-to-charge ratio (/)-resolved imaging for the comprehensive study of the higher-order molecular structure of macromolecular assemblies (MMAs). A bespoke TOF analyzer has been coupled to the higher-energy collisional dissociation cell of an ultrahigh mass range hybrid quadrupole-Orbitrap MS. A 193 nm excimer laser was employed to photofragment MMA ions.
View Article and Find Full Text PDFACS Biomater Sci Eng
June 2023
Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, United States.
Monitoring of extracellular matrix (ECM) microstructure is essential in studying structure-associated cellular processes, improving cellular function, and for ensuring sufficient mechanical integrity in engineered tissues. This paper describes a novel method to study the microscale alignment of the matrix in engineered tissue scaffolds (ETS) that are usually composed of a variety of biomacromolecules derived by cells. First, a trained loading function was derived from Raman spectra of highly aligned native tissue via principal component analysis (PCA), where prominent changes associated with specific Raman bands (e.
View Article and Find Full Text PDFJ Colloid Interface Sci
June 2023
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address:
Anal Chem
September 2022
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.
Core histones including H2A, H2B, H3, and H4 are key modulators of cellular repair, transcription, and replication within eukaryotic cells, playing vital roles in the pathogenesis of disease and cellular responses to environmental stimuli. Traditional mass spectrometry (MS)-based bottom-up and top-down proteomics allows for the comprehensive identification of proteins and of post-translational modification (PTM) harboring proteoforms. However, these methodologies have difficulties preserving near-cellular spatial distributions because they typically require laser capture microdissection (LCM) and advanced sample preparation techniques.
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