The key material for bioethanol production is cellulose, which is one of the main components of the plant cell wall. Enzymatic depolymerization of cellulose is an essential step in bioethanol production, and can be accomplished by fungal and bacterial cellulases. Most of the biochemically characterized bacterial cellulases come from only a few cellulose-degrading bacteria, thus limiting our knowledge of a range of cellulolytic activities that exist in nature. The recent explosion of genomic data offers a unique opportunity to search for novel cellulolytic activities; however, the absence of clear understanding of structural and functional features that are important for reliable computational identification of cellulases precludes their exploration in the genomic datasets. Here, we explore the diversity of cellulases and propose a genomic approach to overcome this bottleneck.
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http://dx.doi.org/10.1016/j.tibtech.2011.04.008 | DOI Listing |
Foods
December 2024
Research Group on Biomass Energy, Department of Nuclear Energy, Federal University of Pernambuco, Recife 50740-545, PE, Brazil.
Food waste (FW) is a common source of contamination, contaminating both soils and water bodies by releasing greenhouse gases. FW holds great potential for biofuel and bioproduct production, which can mitigate its environmental impact and become a valuable addition to the circular bioeconomy. Therefore, this work aimed to investigate the use of food waste as a substrate to produce fermentable sugars and bioethanol.
View Article and Find Full Text PDFFEBS J
January 2025
Department of Biosciences and Bioengineering, IIT Bombay, Mumbai, India.
Cellulases are an ensemble of enzymes that hydrolyze cellulose chains into fermentable glucose and hence are widely used in bioethanol production. The last enzyme of the cellulose degradation pathway, β-glucosidase, is inhibited by its product, glucose. The product inhibition by glucose hinders cellulose hydrolysis limiting the saccharification during bioethanol production.
View Article and Find Full Text PDFBMC Plant Biol
January 2025
Misión Biológica de Galicia (CSIC), Depto. Producción Vegetal, Pazo de Salcedo, Carballeira 8, Pontevedra, 36143, Spain.
Background: The crosslinking of maize cell wall components, particularly mediated by the formation of ferulic acid dimers or diferulates, has been associated with important crop valorization traits such as increased pest resistance, lower forage digestibility, or reduced bioethanol production. However, these relationships were based on studies performed using diverse unrelated inbred lines and/or populations, so genetic background could interfere on these associations.
Results: In the present research, the success of a pedigree selection program aimed to obtain inbred lines from a common antecessor with contrasting diferulate concentration was evaluated.
J Environ Manage
January 2025
School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
The cultivation of edible mushrooms plays a significant role in revitalizing numerous rural regions in China. However, this process generates a large amount of spent mushroom substrate (SMS). Traditional methods for handling SMS, such as random stacking and incineration, lead to resource waste and environmental pollution.
View Article and Find Full Text PDFBraz J Microbiol
December 2024
Laboratory of Yeast Biochemistry (LabBioLev), Federal University of Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil.
This study aimed to compare the effects of cellobiose hydrolysis, whether occurring inside or outside the cell, on the ability of Saccharomyces cerevisiae strains to ferment this sugar and then apply the most effective strategy to industrial S. cerevisiae strains. Firstly, two recombinant laboratory S.
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