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Enzymatic properties of recombinant kojibiose phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494. | LitMetric
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Enzymatic properties of recombinant kojibiose phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494.

Takuo Yamamoto Mio Nishio-Kosaka Seisuke Izawa Hajime Aga Tomoyuki Nishimoto Hiroto Chaen Shigeharu Fukuda

Biosci Biotechnol Biochem

Glycoscience Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., Naka-ku, Okayama, Japan.

Published: October 2011

One kojibiose phoshorylase (KP) homolog gene was cloned from Caldicellulosiruptor saccharolyticus ATCC43494. Recombinant KP from C. saccharolyticus (Cs-KP) expressed in Escherichia coli showed highest activity at pH 6.0 at 85 °C, and was stable from pH 3.5 to 10.0 and up to 85 °C for phosphorolysis. Cs-KP showed higher productivity of kojioligosaccharides of DP ≧ 4 than KP from Thermoanaerobacter brockii ATCC35047.

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http://dx.doi.org/10.1271/bbb.110116DOI Listing

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