In mice, the number of intestinal villous columnar epithelium cells that incorporate abnormal prion protein (PrP(Sc) ) decreases significantly after weaning. In this study, the dynamics of PrP(Sc) uptake during the growth of hamsters were investigated by inoculating scrapie 263K agent orally into suckling and weanling Syrian hamsters and estimating the number of PrP(Sc) -positive villous epithelium cells immunohistochemically. The number of PrP(Sc) -positive cells declined significantly as the hamsters aged. The present results suggest that a tendency toward decline of PrP(Sc) -positive cells with increasing age might be a common phenomenon among the superfamily Muridae.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1348-0421.2011.00359.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative study of immunoassays, a microelectromechanical systems-based biosensor, and RT-QuIC for the diagnosis of chronic wasting disease in white-tailed deer.
BMC Vet Res
November 2024
Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 901 E. Campus Loop, Columbia, MO, USA.
Background: Chronic wasting disease (CWD) is a fatal transmissible spongiform encephalopathy in cervids. The disease is caused by a pathogenic prion, namely PrP. Currently, diagnosis of CWD relies on IHC detection of PrP in the obex or retropharyngeal lymph nodes (RPLN) or ELISA screening of obex and RPLN followed by IHC confirmation of positive results.
View Article and Find Full Text PDFAberrance of GAP43/p-GAP43 Closely Associates with the Pathology of Neuron Loss in Prion-Infected Rodent Models.
Mol Neurobiol
October 2024
National Key-Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, NHC Key Laboratory of Medical Virology and Viral Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Prion diseases are fatal neurodegenerative disorders characterized by neuron damage and loss. Growth-associated protein 43 (GAP43) functions in neuronal plasticity and synaptic function, but its role in prion diseases is not fully elucidated. In this study, we investigated the changes of GAP43 in the central nerve system (CNS) of several prion-infected rodent models and explored the potential relationship of GAP43 with PrP deposit and neuron loss using various methods.
View Article and Find Full Text PDFArticle Synopsis
- - Microglia, crucial cells in the brain, effectively consume prions during early stages of prion disease but shift their role as the disease progresses, moving from phagocytosis to forming direct contacts with neurons.
- - In later stages, microglia surround neurons without fully engulfing them, leading to the accumulation of harmful prions in the brain, while affected neurons show functional decline without cell death markers.
- - This study highlights a novel form of microglial interaction—partial envelopment of neurons—that occurs consistently in various prion diseases, suggesting it is a significant factor in neurodegenerative processes.
Longitudinal detection of prion infection in preclinical sheep blood samples compared using 3 assays.
Blood
October 2024
The Roslin Institute, The Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom.
Variant Creutzfeldt-Jakob disease (vCJD) is a devastating disease caused by transmission of bovine spongiform encephalopathy to humans. Although vCJD cases are now rare, evidence from appendix surveys suggests that a small proportion of the United Kingdom population may be infected without showing signs of disease. These "silent" carriers could present a risk of iatrogenic vCJD transmission through medical procedures or blood/organ donation, and currently there are no validated tests to identify infected asymptomatic individuals using easily accessible samples.
View Article and Find Full Text PDFRT-QuIC detection of chronic wasting disease prion in platelet samples of white-tailed deer.
BMC Vet Res
April 2024
Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, 901 E. Campus Loop, Columbia, MO, USA.
Background: Chronic wasting disease (CWD) is a prion disease of captive and free-ranging cervids. Currently, a definitive diagnosis of CWD relies on immunohistochemistry detection of PrP in the obex and retropharyngeal lymph node (RPLN) of the affected cervids. For high-throughput screening of CWD in wild cervids, RPLN samples are tested by ELISA followed by IHC confirmation of positive results.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!