A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Ultra trace analysis of small molecule by label-free impedimetric immunosensor using multilayer modified electrode. | LitMetric

AI Article Synopsis

  • A multilayer electrode was enhanced with self-assembled thiourea, gold nanoparticles, mercaptosuccinic acid, and antibodies to detect very small amounts of chloramphenicol using an impedimetric system.
  • The optimal modified electrode showed a wide detection range (0.50-10) × 10⁻¹⁶ M and an impressive detection limit of 1.0 × 10⁻¹⁶ M, beating other electrode variants in sensitivity.
  • The electrode demonstrated high selectivity for chloramphenicol, could be reused 45 times with minimal error, and provided comparable results to high-performance liquid chromatography in shrimp sample testing.

Article Abstract

A multilayer electrode modified with a self-assembled thiourea monolayer (SATUM) followed by gold nanoparticles (AuNPs), mercaptosuccinic acid (MSA) and antibody was investigated for the detection of ultra trace amount of a small molecule (chloramphenicol) in an impedimetric system. The formation of the antibody-antigen complex at the electrode surface caused the impedance to increase. Under optimum conditions three modified electrodes were compared the SATUM/AuNPs/MSA electrode provided a wide linear range (0.50-10) × 10⁻¹⁶ M, and a very low determination limit of 1.0 × 10⁻¹⁶ M. This determination limit was much lower than the SATUM/AuNPs electrode, 1.0 × 10⁻¹⁵ M, and SATUM electrode, 4.7 × 10⁻¹⁴ M. The modified electrode provided good selectivity for chloramphenicol detection and can be reused up to 45 times with a relative standard deviation of lower than 4%. When applied to determine chloramphenicol in shrimp samples, the results agreed well with those obtained by the high-performance liquid chromatography coupled with a photo diode array detector (P > 0.05). The developed system can be applied to detect other small molecules using appropriate affinity binding pairs.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2011.05.029DOI Listing

Publication Analysis

Top Keywords

ultra trace
8
small molecule
8
modified electrode
8
electrode provided
8
determination limit
8
electrode
7
trace analysis
4
analysis small
4
molecule label-free
4
label-free impedimetric
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: