Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of an SPR sensor that was first modified with 1-octadecanethiol. A concentration of CGI-I equal to 4.0 μg·mL-1 and a pH of 8.0 were found to give the best results. The dynamic response of the sensor ranges from 0.25 to 1.5 ng·mL-1, and the detection limit is 0.12 ng·mL-1. The sensor was applied to detect CatG in human saliva and white blood cells.FigureThe synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of cathepsin G via a newly developed surface plasmon resonance imaging assay. The sensor was applied to detect cathepsin G in human saliva and white blood cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3092066 | PMC |
| http://dx.doi.org/10.1007/s00604-011-0569-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Staining Susceptibility and Surface Roughness of Teeth Restored by Microabrasion and Resin Infiltration: An In Vitro Study.
Polymers (Basel)
December 2024
Department of Restorative Dentistry, Faculty of Dentistry, Thammasat University, 99 M. 18, Paholyothin Road, Klong Luang, Pathum Thani 12120, Thailand.
This study assessed the susceptibility to staining and surface roughness of white-spot lesions (WSLs) treated with resin infiltration (RIT) and microabrasion (MA) under simulated aging through thermocycling in red wine. Seventy-eight extracted human premolars with artificial WSLs were divided into three groups: untreated WSLs (control), RIT-treated (ICON, DMG), and MA-treated (Opalustre, Ultradent). Each group was further split: one subgroup immersed in artificial saliva and the other thermocycled in red wine.
View Article and Find Full Text PDFObjective: This study aims to compare the effectiveness of 2 artificial saliva substitutes (Biotène vs HydraSmile) in the symptomatic management of radiation-induced xerostomia.
Study Design: Randomized double-blind cross-over study.
Setting: Single tertiary care academic institution.
A novel stable biomimetic adhesive coating for functionalization of orthodontic brackets against bacterial colonization and white spot lesions.
BMC Oral Health
January 2025
Oral Technology, University Hospital Bonn, 53111, Bonn, Germany.
Background: This study aimed to evaluate the efficacy of polydopamine (PDA) functionalization on orthodontic brackets in inhibiting biofilm formation and promoting surface bioactivity to buffer the acidity of caries-causing bacteria around orthodontic brackets and prevent demineralization. The stability of the coating in artificial saliva (AS) and distilled water was evaluated, along with its effect on pH changes in simulated body fluid (SBF) and distilled water.
Methods: Maxillary incisor orthodontic brackets underwent PDA functionalization using a dopamine hydrochloride solution following a specific protocol.
Targeted enamel remineralization with mineral-loaded starch particles.
JADA Found Sci
November 2024
GreenMark Biomedical Inc, East Lansing and Ann Arbor, MI.
Background: Noninvasive caries treatments work topically, which may limit efficacy. The authors hypothesized that an alternative approach using mineral-loaded particles designed to target the subsurface of noncavitated caries lesions could be advantageous. This study shows in vitro proof-of-concept.
View Article and Find Full Text PDFDynamics of the oral microbiome during orthodontic treatment and antimicrobial advances for orthodontic appliances.
iScience
December 2024
Department of Orthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices& Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, No.22, Zhongguancun South Avenue, Haidian District, Beijing 100081, P.R. China.
The oral microbiome plays an important role in human health, and an imbalance of the oral microbiome could lead to oral and systemic diseases. Orthodontic treatment is an effective method to correct malocclusion. However, it is associated with many adverse effects, including white spot lesions, caries, gingivitis, periodontitis, halitosis, and even some systematic diseases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!