AI Article Synopsis
- Ribonucleoside diphosphate reductase (RNR) is concentrated in specific areas within E. coli cells, and the number of these areas increases with more replication cycles.
- As the growth rate of the bacteria increases, the ratio of replication forks to RNR protein foci also increases, indicating a tighter association between these proteins and the replication process.
- Analysis shows that while the total amount of RNR per focus increases with faster growth, the amount of RNR per replication fork remains constant regardless of how many forks are present.
Article Abstract
Ribonucleoside diphosphate reductase (RNR) is located in discrete foci in a number that increases with the overlapping of replication cycles in Escherichia coli. Comparison of the numbers of RNR, DnaX and SeqA protein foci with the number of replication forks at different growth rates reveals that fork : focus ratios augment with increasing growth rates, suggesting a higher cohesion of the three protein foci with increasing number of forks per cell. Quantification of NrdB and SeqA proteins per cell showed: (i) a higher amount of RNR per focus at faster growth rates, which sustains the higher cohesion of RNR foci with higher numbers of forks per cell; and (ii) an equivalent amount of RNR per replication fork, independent of the number of the latter.
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| http://dx.doi.org/10.1099/mic.0.049478-0 | DOI Listing |
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