Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories.

J Clin Microbiol

Molecular Microbiology Laboratory, Mayo Medical Laboratories New England, 160 Dascomb Rd., Andover, MA 01810, USA.

Published: August 2011

This study measured the precision of a quantitative laboratory-developed real-time PCR test for cytomegalovirus performed at three different clinical laboratories that use the same methodology. The overall standard deviation (adjusted for analyte level) was 0.18 log(10) copies/ml, and there was no significant relationship between standard deviation and analytical measuring range.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147729PMC
http://dx.doi.org/10.1128/JCM.00486-11DOI Listing

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