Ethnopharmacological Relevance: Medicinal plants from the Caatinga, a Brazilian xeric shrubland, are used in folk medicine to treat infections. These ethnopharmacological data can contribute to obtaining new antimicrobial/antibiofilm extracts and natural product prototypes for the development of new drugs. The aim of this study was to investigate the antibiofilm and antibacterial activities of 45 aqueous extracts from 24 Caatinga plant species.
Materials And Methods: The effect of aqueous extracts on planktonic cells and on biofilm formation by Staphylococcus epidermidis was studied by the OD(600) absorbance and by the crystal violet assay, respectively. Scanning electron microscopy (SEM) was used to generate comparative images of extract-treated and untreated biofilms. Chromatographic analyses were performed to characterize the active extracts.
Results: The in vitro screening, at 0.4 mg/mL and 4.0mg/mL, showed 20 plants effective in preventing biofilm formation and 13 plants able to inhibit planktonic bacterial growth. SEM images demonstrated distinct profiles of bacterial adhesion, matrix production and cell morphology according to different treatments and surfaces. The phytochemical analysis of the selected active extracts indicates the polyphenols, coumarins, steroids and terpenes as possible active compounds.
Conclusion: This study describes the first antibiofilm and antibacterial screening of Caatinga plants against S. epidermidis. The evaluation presented in this study confirms several ethnopharmacological reports and can be utilized to identify new antibiofilm and antibacterial products against S. epidermidis from traditional Brazilian medicine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2011.05.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone.
AMB Express
January 2025
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 72388, Sakaka, Saudi Arabia.
Coconut oil is eatable oil with many nutritional and cosmetic applications. In this investigation coconut oil was subjected to 0 to 5 L/min of ozone for 3 h and the chemical composition of both crude and ozonized oil was valued via Gas Chromatography-Mass Spectrometry (GC-MS). Some biological tests were done including antibacterial action versus Helicobacter pylori, anti-biofilm activity versus H.
View Article and Find Full Text PDFThe appropriate nutrient conditions for methicillin-resistant Staphylococcus aureus and Candida albicans dual-species biofilm formation in vitro.
Sci Rep
January 2025
Department of Biological and Medical Sciences, Charles University, Faculty of Pharmacy in Hradec Králové, 2089, Zborovská, Hradec Králové, 500 03, Czech Republic.
Polymicrobial biofilms, the reason for most chronic wound infections, play a significant role in increasing antibiotic resistance. The in vivo effectiveness of the new anti-biofilm therapy is conditioned by the profound evaluation using appropriate in vitro biofilm models. Since nutrient availability is crucial for in vitro biofilm formation, this study is focused on the impact of four selected cultivation media on the properties of methicillin-resistant Staphylococcus aureus and Candida albicans dual-species biofilms.
View Article and Find Full Text PDFA thermosensitive chitin hydrogel with mild photothermal-chemotherapy for facilitating multidrug-resistant bacteria infected wound healing.
Int J Biol Macromol
December 2024
Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province & Institute of Advanced Materials and Nanotechnology, School of Chemistry and Chemical Engineering, School of Medicine, Wuhan University of Science and Technology, Wuhan, China.
Bacterial infection of skin wounds leads to serious health problems, including skin defects, inflammatory pain, and even death. To meet the requirements for successful treatment of complicated wounds, a multifunctional dressing is thus highly desirable. In this work, a thermosensitive hydrogel dressing (HBCA) exhibiting injectability, adaptiveness and mild photothermal antibacterial activity was developed for effective infected wound treatment.
View Article and Find Full Text PDFProteomic study of the inhibitory effects of tannic acid on MRSA biofilm.
Front Pharmacol
December 2024
School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, China.
Introduction: The mechanism of tannic acid (TA) intervention on methicillin-resistant (MRSA, USA 300) biofilm formation was explored using proteomics.
Methods: The minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial dilution of the microbroth. The effects of TA were studied using crystal violet staining.
Preparation and Characterization of Carbon Nanodots from turmeric soot for anti-coliform and anti-oral bacterial applications and as anti-staphylococcal coatings.
Nanotheranostics
January 2025
Department of Research and Innovation, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai - 602105, Tamil Nadu, India.
In an era where chemical synthesis of nanomaterial is accounting for the generation of toxic wastes, leading to nanotoxicity, the present work focuses on the extraction of carbon nanodots from available natural sources such as turmeric smoke. The extracted carbon nanodots were characterized and their physical and chemical attributes were confirmed. The antibacterial property of the isolated carbon nanodots was tested against coliforms and oral bacteria.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!