This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441826 | PMC |
| http://dx.doi.org/10.1016/j.carres.2011.05.005 | DOI Listing |
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