A novel stationary phase for weak cation exchange (WCX) and hydrophilic interaction chromatography (HILIC) was prepared with surface-initiated atom transfer radical polymerization (SI-ATRP). Vinyltetrazole was grafted onto the surface of the beads in water medium with the polyglycidylmethacrylate beads (P(GMA/EDMA)) previously modified with 2-bromoisobutryl bromide as the macromolecule initiators and CuCl as catalyst. The poly(vinyltetrazole)-grafted beads obtained with different atom transfer radical polymerization (ATRP) formulations were tried as chromatographic packings in ion-exchange chromatography. The results showed that the prepared columns could separate the tested proteins with high efficiency and high capacity, and the retention time of protein had a positive relationship with increasing the chain lengths of the grafted poly(vinyltetrazole) (PVT). The prepared column was also found to be able to separate nucleosides by hydrophilic interaction chromatographic mode.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2011.04.010DOI Listing

Publication Analysis

Top Keywords

atom transfer
12
transfer radical
12
radical polymerization
12
hydrophilic interaction
12
surface-initiated atom
8
weak cation
8
cation exchange
8
interaction chromatography
8
preparation polyvinyltetrazole
4
polyvinyltetrazole chain-grafted
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!