AI Article Synopsis
- - The study aimed to analyze the exons 2-4 of a new human leukocyte antigen (HLA) allele, specifically HLA-B*15:129.
- - DNA was extracted from a blood sample and amplified using PCR, followed by sequencing, which revealed the presence of both a known allele (B*07:02) and the novel allele.
- - The novel allele, B*15:129, was found to have three nucleotide differences compared to a closely related allele, leading to a specific amino acid change, and has been documented in GenBank.
Article Abstract
Objective: To analyze the sequence of the exons 2-4 of human leukocyte antigen (HLA) novel allele HLA-B*15:129.
Methods: DNA of the proband was extracted from whole blood by commercial DNA extraction kit. The amplification for HLA-B exons 2-4 was performed separately by polymerase chain reaction (PCR) with allele group specific primers. The PCR products were digested with enzymes and then directly sequenced for exons 2-4 of HLA-B locus in both directions.
Results: Sequencing results showed the HLA-B alleles of the proband included B*07:02 and a novel allele. The sequence of the novel allele has been submitted to GenBank (accession no. EF473219) and the allele has been officially named B*15:129 by the WHO Nomenclature Committee. Comparing with the HLA-B*15:01:01:01, the sequence of exons 2-4 of HLA-B*15:129 showed three nucleotide difference in exon 3 at positions 362 and 363 from GG to AT and positions 369 from C to T, which resulted in an amino acid change from Arg to Asn at codon 97.
Conclusion: A novel HLA-B allele was identified and has been officially named B15:129 by the WHO Nomenclature Committee.
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| http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2011.03.012 | DOI Listing |
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