Talanta
Graduate School of Natural Science and Technology (Department of Chemistry, Faculty of Science), Okayama University, 3-1-1 Tsushimanaka, Kitaku, Okayama 700-8530, Japan.
Published: June 2011
A novel reagent, 2',7'-dichlorofluorescein di-tert-butyldimethylsilyl ether (FCl(2)TBS), was synthesized for fluoride ion and used for a sensitive fluorimetric flow injection analysis by detecting the recovery of fluorescence due to cleavage of Si-O bond. Four kinds of fluorescein di-tert-butyldimethylsilyl ether (FTBS) analogues were synthesized and FCl(2)TBS was the best. By introducing chlorine to FTBS, stability of the reagent, reactivity and the baseline signals were improved. The FIA system was three lines. The sample solution (aqua medium) was injected in the carrier solution (water) and merged with the reagent solution (2.0 × 10(-5)M FCl(2)TBS acetone solution), then mixed with phosphate buffer solution (pH 7.5). The fluorescence intensities were measured at λ(ex) 503 nm and λ(em) 527 nm. The calibration graph had linear relationship between (1.0-50.0) × 10(-6)M and the determinable limit was 1.0 × 10(-6)M. The relative standard deviation of 12 measurements with 1.0 × 10(-5)M F(-) solution was 1.0% and the sample throughput was 13 h(-1). The developed method was successfully applied to river and tap water samples.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.talanta.2011.03.081 | DOI Listing |
Talanta
June 2011
Graduate School of Natural Science and Technology (Department of Chemistry, Faculty of Science), Okayama University, 3-1-1 Tsushimanaka, Kitaku, Okayama 700-8530, Japan.
A novel reagent, 2',7'-dichlorofluorescein di-tert-butyldimethylsilyl ether (FCl(2)TBS), was synthesized for fluoride ion and used for a sensitive fluorimetric flow injection analysis by detecting the recovery of fluorescence due to cleavage of Si-O bond. Four kinds of fluorescein di-tert-butyldimethylsilyl ether (FTBS) analogues were synthesized and FCl(2)TBS was the best. By introducing chlorine to FTBS, stability of the reagent, reactivity and the baseline signals were improved.
View Article and Find Full Text PDFSteroids
April 2011
Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland.
A short and efficient synthesis of steroid synthons, di(tert-butyldimethylsilyl) ethers of 3,21-dihydroxy-24-nor-chol-5-en-23-al (8 and 10) and of ethyl 3,21-dihydroxy-25-homo-chola-5,23-dien-25-oate (9 and 11), having natural (20R) and unnatural (20S) configuration from 3β-(tert-butyldimethylsilyloxy)-14α,20ξ-card-5-enolide (2) is reported. Further elongation of the side chain of these synthons provides a new method for the synthesis of (20R) and (20S)-21-hydroxy steroids. The utility of the method was exemplified by the synthesis of a natural marine sterol - 21-hydroxycholesterol (18).
View Article and Find Full Text PDFJ Fluoresc
January 2007
Department of Chemistry, Northwest University, Xi'an 710069, P. R. China.
A highly sensitive and selective fluorogenic probe for fluoride ion, fluorescein di-tert-butyldimethylsilyl ether (FTBS), was designed and synthesized. FTBS was a colorless, non-fluorescent compound and was synthesized via the one-step reaction of fluorescein with tert-butyldimethylsilyl chloride. Upon incubation with fluoride ion in DMF-water solution (7 : 3, V/V), the Si-O bond of FTBS was cleaved, causing a large increase in fluorescence intensity and thereby allowing a selective detection of fluoride ion.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
April 1993
Section on Analytical Biochemistry, Laboratory of Clinical Science, National Institute of Mental Health, Building 10, Room 3D40, NIH, 20892, Bethesda, MD.
A technique for the measurement of thymine glycol at parts per million concentrations in double-stranded polymeric DNA is described. The procedure utilizes base to ring-open DNA-bound thymine glycol in the presence of monomeric [(2)H4]thymine glycol as an internal standard, followed by reduction, solvolytic cleavage, and quantification of the characteristic methyl-2-methylglycerate released from polymeric DNA. Methyl-2-methyl-glycerate is derivatized to form the di-tert-butyldimethylsilyl [(TBDMS)2] ether to enhance its gas chromatographic properties and electron ionization detection.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.