AI Article Synopsis
- The experiment focuses on using thin layer chromatography (TLC) to analyze lipids extracted from simulated amniotic fluid samples.
- It involves separating and detecting lecithin and sphingomyelin, with the calculation of their ratio as a key outcome.
- The lecithin-to-sphingomyelin ratio has clinical significance, and the procedure uses widely available commercial kits for ease of access in medical labs.
Article Abstract
A thin layer chromatography experiment of medical importance is described. The experiment involves extraction of lipids from simulated amniotic fluid samples followed by separation, detection, and scanning of the lecithin and sphingomyelin bands on TLC plates. The lecithin-to-sphingomyelin ratio is calculated. The clinical significance of this number is discussed. Since this is a procedure that is often performed in medical laboratories, most of the supplies and materials for the experiment are commercially available in kit form.
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| http://dx.doi.org/10.1002/bmb.2006.49403401044 | DOI Listing |
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