Informative microsatellites for genetic population studies of black-faced lion tamarins (Leontopithecus caissara).

Genet Mol Biol

Laboratório de Biodiversidade Molecular e Conservação, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil.

Published: January 2011

AI Article Synopsis

  • Leontopithecus caissara is a critically endangered primate from the Brazilian Atlantic Forest, and a study was conducted using 34 individuals from Superagüi Island.
  • Of the 19 microsatellite loci tested, 17 produced strong genetic markers, with 9 of those being polymorphic, resulting in a total of 23 alleles and diverse genetic variation.
  • The study revealed the potential benefits and limitations of using these microsatellite loci for genetic analysis of L. caissara, and suggested their possible application for other related species.

Article Abstract

Leontopithecus caissara is a critically endangered primate species from the Brazilian Atlantic Forest. Nineteen microsatellite loci, previously developed for congeneric species, were tested with 34 L. caissara individuals from Superagüi Island. Of the 19 loci, 17 (89.4%) produced robust alleles, nine (47.4%) of these proved to be polymorphic, with a total of 23 alleles and an average of 2.56 alleles per locus. Expected and observed heterozygosity averaged 0.483 and 0.561, respectively. The exclusion power for identifying the first parent of an arbitrary offspring was 0.315 over all loci. The results thus indicate both the usefulness and limitations of these nine microsatellite loci in the genetic analysis of L. caissara, as well as their potentiality for genetic investigation in other congeneric species.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085366PMC
http://dx.doi.org/10.1590/S1415-47572010005000111DOI Listing

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