Using the MBB/11/5 strain originally adapted to the PLC/PRF/5 cell line, reproduction of the virus in diploid cells of human embryo fibroblasts, continuous primate lines (RAMT, FRhK-4) and human urinary bladder tumor (T-24) lines was studied. We obtained HAV DNA sequences (about 99% from vRNA) for the MBB/11/5 strain which were used as a probe for demonstration of vRNA synthesis in the infected cells.
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Comparison of two defective hepatitis A virus strains adapted to cell cultures.
Acta Virol
May 1992
Institute of Preventive and Clinical Medicine, Bratislava, Czechoslovakia.
The replication of two defective hepatitis A virus strains in cell culture was examined. The w.t.
View Article and Find Full Text PDFA comparative analysis of reproduction of 9 strains of hepatitis A viruses (HSA-15, CF-979, MI, MBB 11/5, H-141, KMW-1, GBM, IH-26, WR-61) from different regions of the world in cell cultures (PLC/PRF/5, HEL-240, FRhK-4, MK) revealed the differences in the capacity of the viruses for reproduction in these cell lines. The factors influencing HAV reproduction in cell cultures such as temperature, medium, and sera were studied. In one-cycle infection, accumulation of vRNA and formation of virus particles were analysed.
View Article and Find Full Text PDFUsing the MBB/11/5 strain originally adapted to the PLC/PRF/5 cell line, reproduction of the virus in diploid cells of human embryo fibroblasts, continuous primate lines (RAMT, FRhK-4) and human urinary bladder tumor (T-24) lines was studied. We obtained HAV DNA sequences (about 99% from vRNA) for the MBB/11/5 strain which were used as a probe for demonstration of vRNA synthesis in the infected cells.
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